Carbonic Anhydrase 8/CA8 Active Protein | CA8 active protein

Carbonic Anhydrase 8/CA8 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120291

• Gene Names: CA8; CALS; CARP; CAMRQ3; CA-VIII
• Purity: >94% as determined by SDS-PAGE
• Synonyms: Carbonic Anhydrase 8/CA8; Carbonic Anhydrase 8/CA8 Protein; Human; Recombinant (His Tag); Human Carbonic Anhydrase VIII/CA8 Protein (His Tag); CA-VIII Protein; CALS Protein; CAMRQ3 Protein; CARP Protein; MGC120502 Protein; MGC99509 Protein; carbonic anhydrase VIII; CA8 active protein

• Host: E Coli
• Purity/Purification: >94% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 15% glycerol, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gln290

• Species: Human
• Activity: Measured by its esterase activity. The specific activity is >100pmoles/min/ug.
• Predicted N Terminal: Met 1
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human CA8 (NP_004047.3)(Met 1-Gln 290) was expressed, with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CA8 active protein

Background: The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer.

References

Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1): 1-20.Miyaji E, et al. (2003) Overexpression of carbonic anhydrase-related protein VIII in human colorectal cancer. The Journal of Pathology. 201(1): 37-45.

NCBI and Uniprot Product Information

• NCBI GI #: 22027500
• NCBI GeneID: 767
• NCBI Accession #: NP_004047.3
• NCBI GenBank Nucleotide #: NM_004056.4
• UniProt Accession #: P35219; Q32MY2; A8K0A5; B3KQZ7
• Molecular Weight: 32,973 Da
• NCBI Official Full Name: carbonic anhydrase-related protein
• NCBI Official Synonym Full Names: carbonic anhydrase VIII
• NCBI Official Symbol: CA8
• NCBI Official Synonym Symbols: CALS; CARP; CAMRQ3; CA-VIII
• NCBI Protein Information: carbonic anhydrase-related protein; CA-related protein; carbonate dehydratase; carbonic anhydrase-like sequence
• UniProt Protein Name: Carbonic anhydrase-related protein
• Protein Family: Carbonic anhydrase-related protein
• UniProt Gene Name: CA8
• UniProt Synonym Gene Names: CALS; CARP; CA-VIII
• UniProt Entry Name: CAH8_HUMAN

NCBI Description

The protein encoded by this gene was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, the gene product lacks carbonic anhydrase activity (i.e., the reversible hydration of carbon dioxide). The gene product continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. The absence of CA8 gene transcription in the cerebellum of the lurcher mutant in mice with a neurologic defect suggests an important role for this acatalytic form. [provided by RefSeq, Jul 2008]

Uniprot Description

CA8: Does not have a carbonic anhydrase catalytic activity. Defects in CA8 are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 3 (CMARQ3). CMARQ3 is a congenital cerebellar ataxia associated with dysarthia, quadrupedal gait and mild mental retardation. Belongs to the alpha-carbonic anhydrase family.

Protein type: Energy Metabolism - nitrogen; Lyase

Chromosomal Location of Human Ortholog: 8q12.1

Cellular Component: cytoplasm

Molecular Function: carbonate dehydratase activity; zinc ion binding

Biological Process: phosphoinositide-mediated signaling; one-carbon compound metabolic process

Disease: Cerebellar Ataxia, Mental Retardation, And Dysequilibrium Syndrome 3