Testing Data
Mycoplasma Arginine Deiminase Active Protein | ADI active protein
Mycoplasma Arginine Deiminase
Purity
>98% by SDS-PAGE gel and HPLC analyses.
Synonyms
Mycoplasma Arginine Deiminase; Recombinant Mycoplasma Arginine Deiminase (ADI); ADI active protein
Host
E Coli
Purity/Purification
>98% by SDS-PAGE gel and HPLC analyses.
Form/Format
Sterile filtered through a 0.2 micron filter. Lyophilized from 2mM Sodium Phosphate, pH 7.5+ 0.2mM DTT.
Sequence
SVFDSKFKG IHVYSEIGE LESVLVHEP GREIDYITP ARLDELLFS AILESHDAR KEHKQFVAE LKANDINVV ELIDLVAET YDLASQEAK DKLIEEFLE DSEPVLSEE HKVVVRNFL KAKKTSREL VEIMMAGIT KTDLGIEAD HELIVDPMP NLYFTRDPF ASVGNGVTI HYMRYKVRQ RETLFSRFV FSNHPKLIN TPWYYDPSL KLSIEGGDV FIYNNDTLV VGVSERTDL QTVTLLAKN IVANKECEF KRIVAINVP KWTNLMHLD TWLTMLDKD KFLYSPIAN DVFKFWDYD LVNGGAEPQ PVENGLPLE GLLQSIINK KPVLIPIAG EGASQMEIE RETHFDGTN YLAIRPGVV IGYSRNEKT NAALEAAGI KVLPFHGNQ LSLGMGNAR CMSMPLSRK DVKW
Authenticity
Verified by N-Terminal and Mass Spectrometry analyses (when applicable).
Endotoxin
Endotoxin level is <0.1 ng/ug of protein (<1EU/ug) .
Protein Content
Verified by UV Sprectroscopy and/or SDS-PAGE gel.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1- 1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Preparation and Storage
At -20 degree C
Testing Data
Testing Data
Related Product Information for ADI active protein
Arginine Deiminase (ADI) is a microbial enzyme from Mycoplasma produced in E Coli. It has high affinity to L-arginine and hydrolyzes L-arginine to citrulline and ammonia. Low concentrations of ADI have been shown to inhibit proliferation in certain cultured cells by arresting the cell cycle in G (1) and/or S phase. Higher concentrations of ADI lead to subsequent apoptosis. Recombinant Mycoplasma Arginine Deiminase is a 46.3 kDa protein consisting of 409 amino acids.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The ADI arca (Catalog #AAA695866) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: SVFDSKFKG IHVYSEIGE LESVLVHEP GREIDYITP ARLDELLFS AILESHDAR KEHKQFVAE LKANDINVV ELIDLVAET YDLASQEAK DKLIEEFLE DSEPVLSEE HKVVVRNFL KAKKTSREL VEIMMAGIT KTDLGIEAD HELIVDPMP NLYFTRDPF ASVGNGVTI HYMRYKVRQ RETLFSRFV FSNHPKLIN TPWYYDPSL KLSIEGGDV FIYNNDTLV VGVSERTDL QTVTLLAKN IVANKECEF KRIVAINVP KWTNLMHLD TWLTMLDKD KFLYSPIAN DVFKFWDYD LVNGGAEPQ PVENGLPLE GLLQSIINK KPVLIPIAG EGASQMEIE RETHFDGTN YLAIRPGVV IGYSRNEKT NAALEAAGI KVLPFHGNQ LSLGMGNAR CMSMPLSRK DVKW. It is sometimes possible for the material contained within the vial of "Mycoplasma Arginine Deiminase, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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