RANTES active protein

Murine RANTES (CCL5)

Cat. Num. AAA696166

• Gene Names: Ccl5; SISd; Scya5; RANTES; TCP228; MuRantes
• Purity: >=98% by SDS-PAGE gel and HPLC analyses.
• Synonyms: RANTES; Murine RANTES (CCL5); Recombinant Murine RANTES (CCL5); Regulation upon Activation Normal T-cells Express Sequence; CCL5; SIS-delta; RANTES active protein

• Host: E Coli
• Purity/Purification: >=98% by SDS-PAGE gel and HPLC analyses.
• Form/Format: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.
• Sequence: SPYGSDTTP CCFAYLSLA LPRAHVKEY FYTSSKCSN LAVVFVTRR NRQVCANPE KKWVQEYIN YLEMS

• Authenticity: Verified by N-terminal and Mass Spectometry analyses (when applicable)
• Biological Activity: Determined by its ability to chemoattract total human lymphocyte population and total murine T cell population using a concentration range of 1.0-10.0 ng/ml.
• Endotoxin: Endotoxin level is <0.1 ng/ug of protein (<1EU/ug).
• Protein Content: Verified by UV Spectroscopy and /or SDS-PAGE gel.
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL/ Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, It is recommended to furter dilute in a buffer containing a carrier protein example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
• Preparation and Storage: Store at -20°C.

Testing Data

Related Product Information for RANTES active protein

RANTES is a CC-chemokine that can signal through the CCR1, CCR3, CCR5 and US28 (cytomegalovirus receptor) receptors. It is a chemoattractant towards monocytes, memory T cells (CD4+/CD45RO), basophils, and eosinophils. RANTES also has the capability to inhibit certain strains of HIV-1, HIV-2 and simian immunodeficiency virus (SIV). Recombinant murine RANTES is a 7.8 kDa protein containing 68 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.

Product Categories/Family for RANTES active protein

Innate Immunity; Immunology; Angiogenesis; Neurosciences; Chemotaxis

NCBI and Uniprot Product Information

• NCBI GI #: 548926
• NCBI GeneID: 20304
• UniProt Accession #: P30882
• NCBI Official Full Name: C-C motif chemokine 5
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 5
• NCBI Official Symbol: Ccl5
• NCBI Official Synonym Symbols: SISd; Scya5; RANTES; TCP228; MuRantes
• NCBI Protein Information: C-C motif chemokine 5; SIS-delta; small inducible cytokine A5; small-inducible cytokine A5; T-cell-specific protein RANTES
• UniProt Protein Name: C-C motif chemokine 5
• UniProt Gene Name: Ccl5
• UniProt Synonym Gene Names: Scya5
• UniProt Entry Name: CCL5_MOUSE

Uniprot Description

CCL5: Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T- cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils. By mitogens. T-cell and macrophage specific. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted; Chemokine

Cellular Component: extracellular space; cell; cytoplasm; extracellular region; intracellular

Molecular Function: heparin binding; protein homodimerization activity; protein self-association; cytokine activity; receptor signaling protein tyrosine kinase activator activity; phosphoinositide phospholipase C activity; protein kinase activity; CCR1 chemokine receptor binding; chemokine activity; chemokine receptor binding; chemokine receptor antagonist activity; chemoattractant activity; phospholipase activator activity; CCR5 chemokine receptor binding

Biological Process: exocytosis; positive regulation of cell adhesion; positive regulation of osteoclast differentiation; response to toxin; positive regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell migration; chemotaxis; positive regulation of cell-cell adhesion mediated by integrin; protein amino acid phosphorylation; positive regulation of homotypic cell-cell adhesion; pseudopodium formation; leukocyte adhesion; cell-cell signaling; positive chemotaxis; calcium ion transport; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; positive regulation of T cell proliferation; inflammatory response; lymphocyte chemotaxis; protein tetramerization; phospholipase D activation; neutrophil activation; negative regulation of G-protein coupled receptor protein signaling pathway; MAPKKK cascade; positive regulation of cellular biosynthetic process; positive regulation of phosphoinositide 3-kinase cascade; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; cellular protein complex assembly; negative regulation of viral genome replication; positive regulation of tyrosine phosphorylation of STAT protein; response to cytokine stimulus; positive regulation of fever; eosinophil chemotaxis; immune response; positive regulation of neuron differentiation; positive regulation of calcium ion transport; positive regulation of defense response to virus by host; regulation of insulin secretion; positive regulation of phosphorylation; positive regulation of epithelial cell proliferation; regulation of T cell activation; positive regulation of cell migration