Rabbit anti-Human OLR1 Polyclonal Antibody | anti-OLR1 antibody

OLR1 (Oxidized Low Density Lipoprotein Receptor 1, LOX1, LOXIN, SLOX1, CLEC8A, SCARE1) (FITC)

Cat. Num. AAA6430151

• Gene Names: OLR1; LOX1; LOXIN; SLOX1; CLEC8A; SCARE1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: OLR1; Polyclonal Antibody; OLR1 (Oxidized Low Density Lipoprotein Receptor 1; LOX1; LOXIN; SLOX1; CLEC8A; SCARE1) (FITC); anti-OLR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human OLR1.
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-OLR1 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human OLR1.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using OLR1 antibody.)

Product Categories/Family for anti-OLR1 antibody

Antibodies; Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 4505501
• NCBI GeneID: 4973
• NCBI Accession #: NP_002534.1
• NCBI GenBank Nucleotide #: NM_002543.3
• UniProt Accession #: P78380; Q2PP00; Q7Z484; A8K7V9; B4DI48; G3V1I4
• Molecular Weight: 30,959 Da
• NCBI Official Full Name: oxidized low-density lipoprotein receptor 1 isoform 1
• NCBI Official Synonym Full Names: oxidized low density lipoprotein (lectin-like) receptor 1
• NCBI Official Symbol: OLR1
• NCBI Official Synonym Symbols: LOX1; LOXIN; SLOX1; CLEC8A; SCARE1
• NCBI Protein Information: oxidized low-density lipoprotein receptor 1; hLOX-1; ox LDL receptor 1; lectin-type oxidized LDL receptor 1; scavenger receptor class E, member 1; C-type lectin domain family 8 member A; oxidized low-density lipoprotein receptor 1, soluble form
• UniProt Protein Name: Oxidized low-density lipoprotein receptor 1
• Protein Family: Oxidized low-density lipoprotein receptor
• UniProt Gene Name: OLR1
• UniProt Synonym Gene Names: CLEC8A; LOX1; Ox-LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1; hLOX-1
• UniProt Entry Name: OLR1_HUMAN

NCBI Description

This gene encodes a low density lipoprotein receptor that belongs to the C-type lectin superfamily. This gene is regulated through the cyclic AMP signaling pathway. The encoded protein binds, internalizes and degrades oxidized low-density lipoprotein. This protein may be involved in the regulation of Fas-induced apoptosis. This protein may play a role as a scavenger receptor. Mutations of this gene have been associated with atherosclerosis, risk of myocardial infarction, and may modify the risk of Alzheimer's disease. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]

Uniprot Description

OLR1: Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro- oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro- atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram- positive bacteria. Independent association genetic studies have implicated OLR1 gene variants in myocardial infarction susceptibility. OLR1 may be involved in Alzheimer disease (AD). Involvement in AD is however unclear: according to some authors (PubMed:12354387, PubMed:12810610 and PubMed:15976314), variations in OLR1 modify the risk of AD, while according to other (PubMed:15000751 and PubMed:15060104) they do not. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.2-p12.3

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; membrane; integral to plasma membrane; plasma membrane; receptor complex; lipid raft

Molecular Function: low-density lipoprotein receptor activity; protein binding; carbohydrate binding

Biological Process: cell death; receptor-mediated endocytosis; leukocyte adhesion; response to hydrogen peroxide; blood circulation; lipoprotein metabolic process; proteolysis; blood coagulation; inflammatory response; leukocyte migration

Disease: Myocardial Infarction, Susceptibility To