M-CSF active protein

Rat M-CSF

Cat. Num. AAA695864

• Reactivity: Species Reactivity: Rat; Cross Reactivity: Mouse
• Purity: > or = 98% by SOS-PAGE gel and HPLC analyses.
• Synonyms: M-CSF; Rat M-CSF; Macrophage Colony Stimulating Factor; CSF-1; MGI-IM; M-CSF active protein

• Host: E Coli
• Reactivity: Species Reactivity: Rat; Cross Reactivity: Mouse
• Purity/Purification: > or = 98% by SOS-PAGE gel and HPLC analyses.
• Form/Format: Sterile filtered through a 0.2 micron filter. Lyophilized from 5 mM Sodium Phosphate, pH 7.5.
• Sequence: MEVSEHCSHM IGNGHLQILQ QLIDSQMETA CLIEYKFVDQ EQLDDPVCYL KKAFVLVQVI IEETMRFKDN TPNANATERL QELSMKLNSC FIKDYKEQNE ACVQTYKESP LRLLEKIKNF FNETKNFLEK DWNIFSKNCN DSLAKCSSRD VVTKP

• Authenticity: Verified by N-terminal and Mass Spectrometry analyses (when applicable).
• Endotoxin: Endotoxin level is <0.1 ng/ug of protein (<1 EU/ug).
• Protein Content: Verified by UV Spectroscopy and/or SOS-PAGE gel.
• Biological activity: Determined by its ability to stimulate the proliferation of murine M-NFS-60 ce1ls. The expected ED₅₀ is < or = 5.0 ng/ml, corresponding to a specific activity of > or = 2 x 10⁵ units/mg.
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1 % BSA) and store in working aliquots at-20°C to -80°C.
• Preparation and Storage: At -20 degree C

Testing Data

Related Product Information for M-CSF active protein

M-CSF is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness, and male and female fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation. The human is reactive in murine systems, but the murine molecule exhibits no activity on human cells. Recombinant rat M-CSF is a 18.1 kDa homodimeric protein containing two 155 amino acid polypeptide subunits.

Product Categories/Family for M-CSF active protein

Innate Immunity; Stem Cell Research; Cancer; Proliferation and Cell Cycle

NCBI and Uniprot Product Information

• NCBI GI #: 28201782
• NCBI GeneID: 78965
• UniProt Accession #: Q8JZQ0
• NCBI Official Full Name: Macrophage colony-stimulating factor 1
• NCBI Official Synonym Full Names: colony stimulating factor 1 (macrophage)
• NCBI Official Symbol: Csf1
• NCBI Protein Information: macrophage colony-stimulating factor 1; MCSF; CSF-1
• UniProt Protein Name: Macrophage colony-stimulating factor 1
• UniProt Gene Name: Csf1
• UniProt Synonym Gene Names: Csfm; CSF-1; MCSF
• UniProt Entry Name: CSF1_RAT

NCBI Description

plays a role in macrophage formation; involved in osteoclastogenesis and endochondral ossification [RGD, Feb 2006]

Uniprot Description

Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance ().