MCP-2 (CCL8) Active Protein | MCP-2 active protein

Recombinant Human MCP-2 (CCL8)

Cat. Num. AAA4159690

• Gene Names: CCL8; HC14; MCP2; MCP-2; SCYA8; SCYA10
• Purity: >95% as determined by SDS-PAGE
• Synonyms: MCP-2 (CCL8); Recombinant Human MCP-2 (CCL8); Monocyte Chemotactic Protein-2; MCP2; HC14; C-C motif chemokine 8; Recombinant Human Monocyte Chemoattractant Protein-2 (CCL8); MCP-2 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from a 0.2 um filtered 20 mM solution in PBS
• Sequence Length: 99

• Bioactivity: The ED(50) was determined by the dose-dependent proliferation of Jurkat cells and was found to be <0.05ng/mL.
• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Testing Data

Testing Data

Related Product Information for MCP-2 active protein

Monocyte chemotactic protein MCP-2 is a C-C chemokine. It shares over 60% amino acid identity with MCP-1 and MCP-3 and has about 30% identity with other C-C chemokines MIP-1 alpha, RANTES, and MIP-1 beta. MCP-2 is chemotactic for and activates a wide variety of inflammatory cells, and its spectrum of action on leukocytes is similar to that of MCP-3, including monocytes, T lymphocytes, natural killer cells, basophils, mast cells, and eosinophils, but differs from MCP-1, which is not active on eosinophils. MCP-2 has been proposed to interact with multiple C-C chemokine receptors, including those used by MCP-1 and MCP-3. However, since leukocytes express a multiplicity of chemokine receptors with promiscuous binding and functional properties, it is difficult to identify the receptors used by a given ligand on these cells.

NCBI and Uniprot Product Information

• NCBI GI #: 2506280
• NCBI GeneID: 6355
• UniProt Accession #: P80075
• Molecular Weight: 9.0 kDa
• NCBI Official Full Name: C-C motif chemokine 8
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 8
• NCBI Official Symbol: CCL8
• NCBI Official Synonym Symbols: HC14; MCP2; MCP-2; SCYA8; SCYA10
• NCBI Protein Information: C-C motif chemokine 8
• UniProt Protein Name: C-C motif chemokine 8
• UniProt Gene Name: CCL8
• UniProt Synonym Gene Names: MCP2; SCYA10; SCYA8; MCP-2
• UniProt Entry Name: CCL8_HUMAN

NCBI Description

This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL8: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis; Chemokine

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: extracellular space

Molecular Function: heparin binding; chemokine activity; protein kinase activity; phospholipase activator activity

Biological Process: cellular calcium ion homeostasis; positive regulation of catalytic activity; exocytosis; cell-cell signaling; calcium ion transport; response to virus; immune response; chemotaxis; inflammatory response; signal transduction; protein amino acid phosphorylation