MCP-2 (CCL8) Active Protein | MCP-2 active protein

Recombinant Mouse MCP-2 (CCL8)

Cat. Num. AAA4159955

• Gene Names: Ccl8; HC14; Mcp2; MCP-2; Scya8; AB023418; 1810063B20Rik
• Purity: >95% as determined by SDS-PAGE
• Synonyms: MCP-2 (CCL8); Recombinant Mouse MCP-2 (CCL8); C-C motif chemokine 8; Monocyte chemotactic protein 2; MCP2; Small-inducible cytokine A8; Mcp2; Scya8; Recombinant Mouse Monocyte Chemoattractant Protein-2 (CCL8); MCP-2 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from a 0.2 um filtered 20 mM PB solution pH 7.5, 100 mM NaCl.
• Sequence Length: 97

• Bioactivity: The ED(50) was determined by the dose-dependent chemo-attraction human PBMC using a range of 10-100 ng/mL.
• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method.
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Testing Data

Testing Data

Related Product Information for MCP-2 active protein

Monocyte chemotactic protein (MCP-2) is a C-C chemokine. It shares over 60% amino acid identity with MCP-1 and MCP-3 and has about 30% identity with other C-C chemokines MIP-1 alpha, RANTES, and MIP-1 beta. MCP-2 is chemotactic for and activates a wide variety of inflammatory cells. Also, its spectrum of action on leukocytes is similar to that of MCP-3. The leukocytes affected are monocytes, T lymphocytes, natural killer cells, basophils, mast cells, and eosinophils-differs from MCP-1 which is not active on eosinophils. MCP-2 has been proposed to interact with multiple C-C chemokine receptors, including those used by MCP-1 and MCP-3. However, since leukocytes express a multiplicity of chemokine receptors with promiscuous binding and functional properties, it is difficult to identify the receptors used by a given ligand on these cells.

NCBI and Uniprot Product Information

• NCBI GI #: 10946818
• NCBI GeneID: 20307
• NCBI Accession #: NP_067418.1
• NCBI GenBank Nucleotide #: NP_067418.1
• UniProt Accession #: Q9Z121
• Molecular Weight: 9.0 kDa
• NCBI Official Full Name: C-C motif chemokine 8
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 8
• NCBI Official Symbol: Ccl8
• NCBI Official Synonym Symbols: HC14; Mcp2; MCP-2; Scya8; AB023418; 1810063B20Rik
• NCBI Protein Information: C-C motif chemokine 8
• UniProt Protein Name: C-C motif chemokine 8
• UniProt Gene Name: Ccl8
• UniProt Synonym Gene Names: Mcp2; Scya8; MCP-2
• UniProt Entry Name: CCL8_MOUSE

Uniprot Description

CCL8: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Chemokine

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; chemokine activity; cytokine activity

Biological Process: immune response; chemotaxis; inflammatory response; positive regulation of myoblast differentiation