SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
IL-22 active protein
Recombinant IL-22
Gene Names
IL22; TIFa; IL-21; IL-22; ILTIF; IL-TIF; IL-D110; zcyto18; TIFIL-23
Applications
SDS-Page
Purity
>90% by SDS-PAGE
Synonyms
IL-22; Recombinant IL-22; Interleukin-22; IL22; IL-21; IL-D110; IL-TIF; ILTIF; TIFa; TIFIL-23; zcyto18; IL-22 active protein
Host
Baculovirus
Purity/Purification
>90% by SDS-PAGE
Form/Format
Liquid; In Phosphate Buffered Saline (pH 7.4) Containing 10% Glycerol.
Concentration
1.0mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence Positions
34-179aa
Sequence
ADPAPISSHC RLDKSNFQQP YITNRTFMLA KEASLADNNT DVRLIGEKLF HGVSMSERCY LMKQVLNFTL EEVLFPQSDR FQPYMQEVVP FLARLSNRLS TCHIEGDDLH IQRNVQKLKD TVKKLGESGE IKAIGELDLL FMSLRNACIH HHHHH
Sequence Length
179
Applicable Applications for IL-22 active protein
Bioactivity, SDS-PAGE
Species
Human
Endotoxin
<1.0 EU per 1ug of protein (determined by LAL method)
Tag
His-Tag
Predicted N-Terminal
Ala
Biological Activity
Measured by its ability to induce IL-10 secretion using COLO 205 human colorectal adenocarcinoma cell. The ED50 for this effects is less or equal to 1.2 ng/ml.
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
Related Product Information for IL-22 active protein
IL22, also known as interleukin-22, is a member of a group of cytokines involved the IL-10 family or IL-10 subfamily. This protein shares us of IL-10R2 in cell signaling with other members of this family, IL-10, IL-26, IL-28A/B and IL-29. It is produced by normal T cells upon anti-CD3 stimulation and initiates innate immune responses against bacterial pathogens especially in epithelial cells such as respiratory and gut epithelial cells. Also, it can be produced by Th17 cells and play a role in the coordinated response of both adaptive and innate immune systems. Biological activity of IL-22 is the functional receptor complex consists of two receptor subunits, IL-22R and IL-10R and is further regulated by interactions with a soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1. Recombinant human IL22, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for IL-22 active protein
References
Kontenko SV., et al, (2001) J. Biol. Chem. 276:2725-2732. Xie MH., et al, (2000) J. Biol. Chem. 275:31335-31339.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17.8kDa (155aa); 18-28kDa (SDS-PAGE under reducing conditions)
NCBI Official Full Name
interleukin-22
NCBI Official Synonym Full Names
interleukin 22
NCBI Official Symbol
IL22
NCBI Official Synonym Symbols
TIFa; IL-21; IL-22; ILTIF; IL-TIF; IL-D110; zcyto18; TIFIL-23
NCBI Protein Information
interleukin-22
UniProt Protein Name
Interleukin-22
UniProt Gene Name
IL22
UniProt Synonym Gene Names
ILTIF; ZCYTO18; IL-22; IL-TIF
UniProt Entry Name
IL22_HUMAN
NCBI Description
This gene is a member of the IL10 family of cytokines that mediate cellular inflammatory responses. The encoded protein functions in antimicrobial defense at mucosal surfaces and in tissue repair. This protein also has pro-inflammatory properties and plays a role in in the pathogenesis of several intestinal diseases. [provided by RefSeq, Jul 2018]
Uniprot Description
IL22: Cytokine that contributes to the inflammatory response in vivo. Belongs to the IL-10 family.
Protein type: Secreted, signal peptide; Cytokine; Secreted
Chromosomal Location of Human Ortholog: 12q15
Cellular Component: extracellular space; extracellular region
Molecular Function: interleukin-22 receptor binding; cytokine activity
Biological Process: cell-cell signaling; response to glucocorticoid stimulus; acute-phase response; inflammatory response
Research Articles on IL-22
Similar Products
Product Notes
The IL-22 il22 (Catalog #AAA201107) is an Active Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 34-179aa. AAA Biotech's IL-22 can be used in a range of immunoassay formats including, but not limited to, Bioactivity, SDS-PAGE. Researchers should empirically determine the suitability of the IL-22 il22 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ADPAPISSHC RLDKSNFQQP YITNRTFMLA KEASLADNNT DVRLIGEKLF HGVSMSERCY LMKQVLNFTL EEVLFPQSDR FQPYMQEVVP FLARLSNRLS TCHIEGDDLH IQRNVQKLKD TVKKLGESGE IKAIGELDLL FMSLRNACIH HHHHH. It is sometimes possible for the material contained within the vial of "IL-22, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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