Rat Glutathione S Transferase Alpha 1 ELISA Kit | GSTA1 elisa kit

Rat Glutathione S Transferase Alpha 1 ELISA Kit

Cat. Num. AAA761749

• Gene Names: GSTA1; GST2; GTH1; GSTA1-1; GST-epsilon
• Reactivity: Rat
• Synonyms: Glutathione S Transferase Alpha 1; Rat Glutathione S Transferase Alpha 1 ELISA Kit; GSTA1; GSTA1 elisa kit

• Reactivity: Rat
• Sequence Length: 222

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for GSTA1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat GSTA1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat GSTA1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat GSTA1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat GSTA1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 31657185
• NCBI GeneID: 2938
• Molecular Weight: 25,631 Da
• NCBI Official Full Name: Glutathione S-transferase alpha 1
• NCBI Official Synonym Full Names: glutathione S-transferase alpha 1
• NCBI Official Symbol: GSTA1
• NCBI Official Synonym Symbols: GST2; GTH1; GSTA1-1; GST-epsilon
• NCBI Protein Information: glutathione S-transferase A1
• UniProt Protein Name: Glutathione S-transferase A1
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: GSTA1
• UniProt Entry Name: GSTA1_HUMAN

NCBI Description

This gene encodes a member of a family of enzymes that function to add glutathione to target electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. This action is an important step in detoxification of these compounds. This subfamily of enzymes has a particular role in protecting cells from reactive oxygen species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. This gene is located in a cluster of similar genes and pseudogenes on chromosome 6. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Uniprot Description

GSTA1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Belongs to the GST superfamily. Alpha family.

Protein type: EC 2.5.1.18; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Other Amino Acids Metabolism - glutathione; Xenobiotic Metabolism - metabolism by cytochrome P450; Transferase

Chromosomal Location of Human Ortholog: 6p12.1

Cellular Component: cytosol

Molecular Function: glutathione transferase activity

Biological Process: epithelial cell differentiation; glutathione metabolic process; metabolic process; xenobiotic metabolic process