Rabbit anti-Human INDO Polyclonal Antibody | anti-INDO antibody

INDO antibody

Cat. Num. AAA9404087

• Gene Names: IDO1; IDO; INDO; IDO-1
• Reactivity: Human
• Applications: Western Blot
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: INDO; Polyclonal Antibody; INDO antibody; CD107B; IDO; anti-INDO antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total INDO antibody.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 403

• Applicable Applications for anti-INDO antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500 - 1:2000
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human INDO.
• Target Name: INDO
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of Placenta cell lines, using INDO antibody.)

Related Product Information for anti-INDO antibody

INDO/IDO1/indoleamine 2,3-dioxygenase (IDO) is an IFN-g-inducible enzyme that catalyzes the rate-limiting step of tryptophan degradation (1). IDO is upregulated in many tumors and in dendritic cells in tumor-draining lymph nodes. Elevated tryptophan catabolism in these cells leads to tryptophan starvation of T cells, limiting T cell proliferation and activation (2). Therefore, IDO is considered an immunosuppresive molecule, and research studies have shown that upregulation of IDO is a mechanism of cancer immune evasion (3). The gastrointestinal stromal tumor drug, imatinib, was found to act, in part, by reducing IDO expression, resulting in increased CD8+ T cell activation and induction of apoptosis in regulatory T cells (4). In addition to its enzymatic activity, IDO was recently shown to have signaling capability through an immunoreceptor tyrosine-based inhibitory motif (ITIM) that is phosphorylated by Fyn in response to TGF-b. This leads to recruitment of SHP-1 and activatation of the noncanonical NF-kB pathway (5).

Product Categories/Family for anti-INDO antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4504577
• NCBI GeneID: 3620
• NCBI Accession #: NP_002155.1
• NCBI GenBank Nucleotide #: NM_002164.5
• UniProt Accession #: P14902; Q540B4
• Molecular Weight: 45,326 Da
• NCBI Official Full Name: indoleamine 2,3-dioxygenase 1
• NCBI Official Synonym Full Names: indoleamine 2,3-dioxygenase 1
• NCBI Official Symbol: IDO1
• NCBI Official Synonym Symbols: IDO; INDO; IDO-1
• NCBI Protein Information: indoleamine 2,3-dioxygenase 1
• UniProt Protein Name: Indoleamine 2,3-dioxygenase 1
• Protein Family: indole-2-monooxygenase
• UniProt Gene Name: IDO1
• UniProt Synonym Gene Names: IDO; INDO; IDO-1
• UniProt Entry Name: I23O1_HUMAN

NCBI Description

This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011]

Uniprot Description

INDO: Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. Belongs to the indoleamine 2,3-dioxygenase family.

Protein type: Amino Acid Metabolism - tryptophan; EC 1.13.11.52; Oxidoreductase; Cell cycle regulation

Chromosomal Location of Human Ortholog: 8p12-p11

Cellular Component: smooth muscle contractile fiber; stereocilium bundle; cytosol

Molecular Function: electron carrier activity; metal ion binding; indoleamine 2,3-dioxygenase activity; heme binding; tryptophan 2,3-dioxygenase activity

Biological Process: regulation of activated T cell proliferation; positive regulation of T-helper 2 type immune response; negative regulation of interleukin-10 production; positive regulation of chronic inflammatory response; positive regulation of interleukin-12 production; tryptophan catabolic process to kynurenine; response to lipopolysaccharide; female pregnancy; negative regulation of T cell proliferation; cytokine production during acute inflammatory response; multicellular organismal response to stress; tryptophan catabolic process; positive regulation of T cell tolerance induction