Rabbit anti-Human NFKBIL1 Polyclonal Antibody | anti-NFKBIL1 antibody

NFKBIL1, ID (NFKBIL1, IKBL, NF-kappa-B inhibitor-like protein 1, Inhibitor of kappa B-like protein, Nuclear factor of kappa light polypeptide gene enhancer in B Cells inhibitor-like 1) (PE)

Cat. Num. AAA6325113

• Gene Names: NFKBIL1; IKBL; NFKBIL
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NFKBIL1; Polyclonal Antibody; ID (NFKBIL1; IKBL; NF-kappa-B inhibitor-like protein 1; Inhibitor of kappa B-like protein; Nuclear factor of kappa light polypeptide gene enhancer in B Cells inhibitor-like 1) (PE); Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 1; anti-NFKBIL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 358

• Applicable Applications for anti-NFKBIL1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IF: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: NFKBIL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256-289 amino acids from the Central region of human NFKBIL1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(NFKBIL1 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the NFKBIL1 antibody detected the NFKBIL1 protein (arrow).)

Flow Cytometry (FC/FACS)

(NFKBIL1 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of NFKBIL1 Antibody (Center) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-NFKBIL1 antibody

This gene encodes a divergent member of the I-kappa-B family of proteins. Its function has not been determined. The gene lies within the major histocompatibility complex (MHC) class I region on chromosome 6. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Product Categories/Family for anti-NFKBIL1 antibody

Antibodies; Protein Kinase Inhibitors

References

Clancy, R.M., et al. Arthritis Rheum. 62(11):3415-3424(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Owecki, M.K., et al. Pol. Merkur. Lekarski 28(167):366-370(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 222352177
• NCBI GeneID: 4795
• NCBI Accession #: NP_001138434.1
• NCBI GenBank Nucleotide #: NP_001138434.1
• UniProt Accession #: Q9UBC1
• NCBI Official Full Name: NF-kappa-B inhibitor-like protein 1 isoform 3
• NCBI Official Synonym Full Names: NFKB inhibitor like 1
• NCBI Official Symbol: NFKBIL1
• NCBI Official Synonym Symbols: IKBL; NFKBIL
• NCBI Protein Information: NF-kappa-B inhibitor-like protein 1
• UniProt Protein Name: NF-kappa-B inhibitor-like protein 1
• Protein Family: NF-kappa-B inhibitor-like protein
• UniProt Gene Name: NFKBIL1
• UniProt Synonym Gene Names: IKBL; I-kappa-B-like protein; IkappaBL

NCBI Description

This gene encodes a divergent member of the I-kappa-B family of proteins. Its function has not been determined. The gene lies within the major histocompatibility complex (MHC) class I region on chromosome 6. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]

Uniprot Description

NFKBIL1: Involved in the regulation of innate immune response. Acts as negative regulator of Toll-like receptor and interferon- regulatory factor (IRF) signaling pathways. Contributes to the negative regulation of transcriptional activation of NF-kappa-B target genes in response to endogenous proinflammatory stimuli. Defects in NFKBIL1 are a cause of susceptibility to rheumatoid arthritis (RA). It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription regulation

Chromosomal Location of Human Ortholog: 6p21.33

Cellular Component: intermediate filament cytoskeleton; nucleoplasm; nucleus

Molecular Function: protein binding

Biological Process: cytoplasmic sequestering of transcription factor; I-kappaB kinase/NF-kappaB cascade; inhibition of NF-kappaB transcription factor; negative regulation of lipopolysaccharide-mediated signaling pathway; negative regulation of toll-like receptor signaling pathway; negative regulation of tumor necrosis factor production

Disease: Rheumatoid Arthritis