GSTA4 active protein

GSTA4, human recombinant

Cat. Num. AAA844345

• Gene Names: GSTA4; GTA4; GSTA4-4
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: GSTA4; human recombinant; Glutathione S-transferase alpha 4; GSTA4-4; GTA4; GSTA4 active protein

• Host: E Coli
• Purity/Purification: >=95%
• Form/Format: 1 mg/ml solution 20 mM Tris-HCl buffer, pH 8.0, 20% glycerol, 2 mM DTT and 100 mM NaCl.; Appearance: Liquid
• Concentration: 1 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAARPK LHYPNGRGRM ESVRWVLAAA GVEFDEEFLE TKEQLYKLQD GNHLLFQQVP MVEIDGMKLV QTRSILHYIA DKHNLFGKNL KERTLIDMYV EGTLDLLELL IMHPFLKPDD QQKEVVNMAQ KAIIRYFPVF EKILRGHGQS FLVGNQLSLA DVILLQTILA LEEKIPNILS AFPFLQEYTV KLSNIPTIKR FLEPGSKKKP PPDEIYVRTV YNIFRP
• Sequence Length: 222

• Applicable Applications for GSTA4 active protein: SDS-PAGE
• Biological Activity: Specific activity > 0.9 units/mg, one unit is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2, 4- dinitrobenzene (CDNB), with reduced glutathione per minute pH 6.5 at 25 degree C.
• Results: Specific activity > 0.9 units/mg
• Unit Definition: One unit is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2, 4- dinitrobenzene (CDNB), with reduced glutathione per minute pH 6.5 at 25 degree C.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for GSTA4 active protein

Background: GSTA4, also known as glutathione S-transferase A4, belongs to the GST superfamily. This enzyme is involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. GSTA4 shows very high activity with reactive carbonyl compounds such as alk-2-enals. GSTA4 is highly effective in catalyzing the conjugate addition of reduced glutathione to 4-hydroxynonenal, an important product of peroxidative degradation of arachidonic acid and a commonly used biomarker for oxidative damage in tissue. This enzyme is expressed at a high level in brain, placenta, and skeletal muscle and much lower in lung and liver. Recombinant human GSTA4 protein, fused to His-tag at N-terminus, was expressed in E.Coli and purified by using conventional chromatography techniques.

Product Categories/Family for GSTA4 active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z) Cell Damage & Oxidative Stress; Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 4504173
• NCBI GeneID: 2941
• NCBI Accession #: NP_001503.1
• NCBI GenBank Nucleotide #: NM_001512.3
• UniProt Accession #: O15217; Q5T7Q8; Q6P4G1; Q9BX18; Q9H414; B2RD15
• Molecular Weight: 28.3 kDa (246 aa, 1-222 aa + His Tag)
• NCBI Official Full Name: glutathione S-transferase A4
• NCBI Official Synonym Full Names: glutathione S-transferase alpha 4
• NCBI Official Symbol: GSTA4
• NCBI Official Synonym Symbols: GTA4; GSTA4-4
• NCBI Protein Information: glutathione S-transferase A4
• UniProt Protein Name: Glutathione S-transferase A4
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: GSTA4
• UniProt Entry Name: GSTA4_HUMAN

NCBI Description

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, which are located in a cluster on chromosome 6, are highly related and encode enzymes with glutathione peroxidase activity that function in the detoxification of lipid peroxidation products. Reactive electrophiles produced by oxidative metabolism have been linked to a number of degenerative diseases including Parkinson's disease, Alzheimer's disease, cataract formation, and atherosclerosis. [provided by RefSeq, Jul 2008]

Uniprot Description

GSTA4: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. This isozyme has a high catalytic efficiency with 4-hydroxyalkenals such as 4- hydroxynonenal (4-HNE). Belongs to the GST superfamily. Alpha family.

Protein type: Transferase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Other Amino Acids Metabolism - glutathione; EC 2.5.1.18; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 6p12.1

Cellular Component: cytosol

Molecular Function: protein homodimerization activity; glutathione transferase activity

Biological Process: glutathione metabolic process; xenobiotic metabolic process