GSTA1 active protein

GSTA1, 1-222aa, Human, E Coli (Bioactivity Validated)

Cat. Num. AAA203481

• Gene Names: GSTA1; GST2; GTH1; GSTA1-1; GST-epsilon
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: GSTA1; 1-222aa; Human; E Coli (Bioactivity Validated); Glutathione S-transferase alpha 1; GST2; GSTA1-1; GTH1; Glutathione S-transferase alpha 1 Glutathione S transferase A1; GST epsilon; GSTA1 1; HA subunit 1.; GSTA1 active protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MAEKPKLHYF NARGRMESTR WLLAAAGVEF EEKFIKSAED LDKLRNDGYL MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKERA LIDMYIEGIA DLGEMILLLP VCPPEEKDAK LALIKEKIKN RYFPAFEKVL KSHGQDYLVG NKLSRADIHL VELLYYVEEL DSSLISSFPL LKALKTRISN LPTVKKFLQP GSPRKPPMDE KSLEEARKIF RF
• Sequence Length: 1-222 aa

• Applicable Applications for GSTA1 active protein: SDS-PAGE, Enzyme Activity
• Domain: 1-222aa
• Antigen Species: Human
• Endotoxin Level: < 1 EU per 1 ug of protein (determined by LAL method)
• Biological Activity: Specific activity is > 35,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.
• Tag: Non-Tagged
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Ships on dry ice. Can be stored at 2-8°C degree C short term 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

Related Product Information for GSTA1 active protein

GSTA1, also known as Glutathione S-transferase alpha 1, belongs to a glutathione S-transferase alpha class and is the most abundantly expressed in liver. This protein functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Recombinant human GSTA1 protein was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for GSTA1 active protein

Enzymes & Proteases

References

Rossi D., et al. (2009) Leukemia. 23 (6):1118-26. ; Hou L., et al. (2007) J Biol Chem. 282 (32):23264-74.;

NCBI and Uniprot Product Information

• NCBI GI #: 22091454
• NCBI GeneID: 2938
• NCBI Accession #: NP_665683
• NCBI GenBank Nucleotide #: NM_145740.4
• UniProt Accession #: P08263; Q14750; Q5GHF8; Q5SZC1
• Molecular Weight: 25.6 kDa (222aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: glutathione S-transferase A1 isoform 1
• NCBI Official Synonym Full Names: glutathione S-transferase alpha 1
• NCBI Official Symbol: GSTA1
• NCBI Official Synonym Symbols: GST2; GTH1; GSTA1-1; GST-epsilon
• NCBI Protein Information: glutathione S-transferase A1
• UniProt Protein Name: Glutathione S-transferase A1
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: GSTA1

NCBI Description

This gene encodes a member of a family of enzymes that function to add glutathione to target electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. This action is an important step in detoxification of these compounds. This subfamily of enzymes has a particular role in protecting cells from reactive oxygen species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. This gene is located in a cluster of similar genes and pseudogenes on chromosome 6. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Uniprot Description

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.