Gpt active protein

Gpt, 1-496 aa, Mouse, His-tag, E Coli (Bioactivity Validated)

Cat. Num. AAA206536

• Gene Names: Gpt; ALT; ALT1; Gpt1; Gpt-1; 1300007J06Rik; 2310022B03Rik
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: Gpt; 1-496 aa; Mouse; His-tag; E Coli (Bioactivity Validated); Alanine aminotransferase 1; ALT; ALT1; Gpt-1; Gpt1; Gpt active protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 1mM DTT, 10% glycerol
• Concentration: 1 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMASQRND RIQASRNGLK GKVLTLDTMN PCVRRVEYAV RGPIVQRALE LEQELRQGVK KPFTEVIRAN IGDAQAMGQR PITFFRQVLA LCVYPNLLSS PDFPEDAKRR AERILQACGG HSLGAYSISS GIQPIREDVA QYIERRDGGI PADPNNIFLS TGASDAIVTM LKLLVAGEGR ARTGVLIPIP QYPLYSAALA ELDAVQVDYY LDEERAWALD IAELRRALCQ ARDRCCPRVL CVINPGNPTG QVQTRECIEA VIRFAFEEGL FLMADEVYQD NVYAEGSQFH SFKKVLTEMG PPYATQQELA SFHSVSKGYM GECGFRGGYV EVVNMDAEVQ KQMAKLMSVR LCPPVPGQAL MGMVVSPPTP SEPSFKQFQA ERQEVLAELA AKAKLTEQVF NEAPGIRCNP VQGAMYSFPQ IQLPLKAVQR AQDLGLAPDM FFCLCLLEET GICVVPGSGF GQQEGTYHFR MTILPPMEKL RVLLEKLRHF HAKFTHEYS
• Sequence Length: 496

• Applicable Applications for Gpt active protein: SDS-PAGE
• Antigen Species: Mouse
• Tag Information: His-tag
• Biological Activity: Specific activity is > 40units/mg, and is defined as the amount of enzyme that convert 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for Gpt active protein

Gpt, also known as alanine aminotransferases 1, catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. This protein plays a key role in the intermediary metabolism of glucose and amino acids. It is widely used as an index of liver integrity or hepatocellular damage in clinical settings. Recombinant mouse Gpt protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for Gpt active protein

Metabolism; Signal Transduction

References

Sohocki MM. et al. (1997) Genomics. 40 (2):247-52.; Matthews CC. et al. (2003) Brain Res. 978 (1-2):59-64.

NCBI and Uniprot Product Information

• NCBI GI #: 33413404
• NCBI GeneID: 76282
• NCBI Accession #: NP_877957
• NCBI GenBank Nucleotide #: NM_182805.3
• UniProt Accession #: Q8QZR5
• Molecular Weight: 57.5 kDa (519 aa) confirmed by MALDI-TOF
• NCBI Official Full Name: alanine aminotransferase 1
• NCBI Official Synonym Full Names: glutamic pyruvic transaminase, soluble
• NCBI Official Symbol: Gpt
• NCBI Official Synonym Symbols: ALT; ALT1; Gpt1; Gpt-1; 1300007J06Rik; 2310022B03Rik
• NCBI Protein Information: alanine aminotransferase 1
• UniProt Protein Name: Alanine aminotransferase 1
• Protein Family: Alanine aminotransferase
• UniProt Gene Name: Gpt
• UniProt Synonym Gene Names: Gpt1; ALT1; GPT 1

Uniprot Description

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles ().