SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
ALT2/GPT2 active protein
Recombinant ALT2/GPT2
Gene Names
Gpt2; ALT2; C87201; AU014768; AU041193; 4631422C05Rik
Applications
SDS-Page
Purity
>85% by SDS-PAGE
Synonyms
ALT2/GPT2; Recombinant ALT2/GPT2; Alanine aminotransferase 2; ALT2; Glutamic--alanine transaminase 2; Glutamic-pyruvic transaminase 2; Glutamate pyruvate transaminase 2; ALT2/GPT2 active protein
Host
E Coli
Purity/Purification
>85% by SDS-PAGE
Form/Format
Liquid; In 20mM Tris-HCl (pH7.5) Containing 20% Glycerol, 2mM DTT.
Concentration
1mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence Positions
1-522aa
Sequence
MGSSHHHHHH SSGLVPRGSH M QRAAVLVRR GSCPRASGPW GRSHSSAAAE ASAALKVRPE RSPRDRILTL ESMNPQVKAV EYAVRGPIVL KAGEIEMELQ RGIKKPFTEV IRANIGDAHA MGQQPITFLR QVMALCTYPN LLNSPSFPED AKKRARRILQ ACGGNSLGSY SASQGVNCIR EDVAAFITRR DGVPADPDNI YLTTGASDGI STILKLLVSG GGKSRTGVMI PIPQYPLYSA VISELDAVQV NYYLDEENCW ALNVDELRRA LRQAKDHCDP KVLCIINPGN PTGQVQSRKC IEDVIHFAWE EKLFLLADEV YQDNVYSPDC RFHSFKKVLY QMGHEYSSNV ELASFHSTSK GYMGECGYRG GYMEVINLHP EIKGQLVKLL SVRLCPPVSG QAAMDIVVNP PEPGEESFEQ FSREKEFVLG NLAKKAKLTE DLFNQVPGIQ CNPLQGAMYA FPRILIPAKA VEAAQSHKMA PDMFYCMKLL EETGICVVPG SGFGQREGTY HFRMTILPPV DKLKTVLHKV KDFHLKFLEQ YS
Sequence Length
522
Applicable Applications for ALT2/GPT2 active protein
SDS-PAGE, Enzyme Activity
Species
Mouse
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Tag
His-Tag
Biological Activity
Specific activity is > 50units/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minuteat pH7.5 at 37C.
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Related Product Information for ALT2/GPT2 active protein
ALT2/GPT2, also known as alanine aminotransferase, catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Subsequently, they play a key role in the intermediary metabolism of glucose and amino acids. Recombinant mouse ALT2/GPT2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for ALT2/GPT2 active protein
References
Sohocki M.M. et al. (1997) Genomics 40(2):247-52. Matthews C.C. et al. (2003) Brain Res. 978(1-2): 59-64.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
60.1kDa (542aa) confirmed by MALDI-TOF
NCBI Official Full Name
alanine aminotransferase 2
NCBI Official Synonym Full Names
glutamic pyruvate transaminase (alanine aminotransferase) 2
NCBI Official Symbol
Gpt2
NCBI Official Synonym Symbols
ALT2; C87201; AU014768; AU041193; 4631422C05Rik
NCBI Protein Information
alanine aminotransferase 2
UniProt Protein Name
Alanine aminotransferase 2
UniProt Gene Name
Gpt2
UniProt Synonym Gene Names
Aat2; ALT2; GPT 2
UniProt Entry Name
ALAT2_MOUSE
Research Articles on ALT2/GPT2
Similar Products
Product Notes
The ALT2/GPT2 gpt2 (Catalog #AAA201146) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-522aa. AAA Biotech's ALT2/GPT2 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Enzyme Activity. Researchers should empirically determine the suitability of the ALT2/GPT2 gpt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH M QRAAVLVRR GSCPRASGPW GRSHSSAAAE ASAALKVRPE RSPRDRILTL ESMNPQVKAV EYAVRGPIVL KAGEIEMELQ RGIKKPFTEV IRANIGDAHA MGQQPITFLR QVMALCTYPN LLNSPSFPED AKKRARRILQ ACGGNSLGSY SASQGVNCIR EDVAAFITRR DGVPADPDNI YLTTGASDGI STILKLLVSG GGKSRTGVMI PIPQYPLYSA VISELDAVQV NYYLDEENCW ALNVDELRRA LRQAKDHCDP KVLCIINPGN PTGQVQSRKC IEDVIHFAWE EKLFLLADEV YQDNVYSPDC RFHSFKKVLY QMGHEYSSNV ELASFHSTSK GYMGECGYRG GYMEVINLHP EIKGQLVKLL SVRLCPPVSG QAAMDIVVNP PEPGEESFEQ FSREKEFVLG NLAKKAKLTE DLFNQVPGIQ CNPLQGAMYA FPRILIPAKA VEAAQSHKMA PDMFYCMKLL EETGICVVPG SGFGQREGTY HFRMTILPPV DKLKTVLHKV KDFHLKFLEQ YS. It is sometimes possible for the material contained within the vial of "ALT2/GPT2, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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