GPT/ALT1 Active Protein | GPT active protein

GPT / ALT1, human recombinant

Cat. Num. AAA844988

• Gene Names: GPT; AAT1; ALT1; GPT1
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: GPT/ALT1; GPT / ALT1; human recombinant; alanine aminotransferase 1; AAT1; ALT1; GPT1; Alanine Transaminase; Alanine aminotransferase 1; ALAT1_HUMAN; Glutamate pyruvate transaminase 1; Glutamic alanine transaminase 1; Glutamic pyruvate transaminase; Glutamic pyruvic transaminase 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1; gpt; GPT 1; GPT active protein

• Host: E. Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid; 0.5 mg/ml solution 20 mM Tris-HCl buffer, pH 8.0, 20% glycerol and 1 mM DTT.
• Sequence Positions: 1-496
• Sequence: MGSSHHHHHH SSGLVPRGSH MASSTGDRSQ AVRHGLRAKV LTLDGMNPRV RRVEYAVRGP IVQRALELEQ ELRQGVKKPF TEVIRANIGD AQAMGQRPIT FLRQVLALCV NPDLLSSPNF PDDAKKRAER ILQACGGHSL GAYSVSSGIQ LIREDVARYI ERRDGGIPAD PNNVFLSTGA SDAIVTVLKL LVAGEGHTRT GVLIPIPQYP LYSATLAELG AVQVDYYLDE ERAWALDVAE LHRALGQARD HCRPRALCVI NPGNPTGQVQ TRECIEAVIR FAFEERLFLL ADEVYQDNVY AAGSQFHSFK KVLMEMGPPY AGQQELASFH STSKGYMGEC GFRGGYVEVV NMDAAVQQQM LKLMSVRLCP PVPGQALLDL VVSPPAPTDP SFAQFQAEKQ AVLAELAAKA KLTEQVFNEA PGISCNPVQG AMYSFPRVQL PPRAVERAQE LGLAPDMFFC LRLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPLEKLRLL LEKLSRFHAK FTLEYS

• Applicable Applications for GPT active protein: SDS-PAGE (SDS)
• Biological Activity: Specific activity is > 20 unit/mg; One unit will convert 1.0 umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37ºC.
• Preparation and Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Testing Data

Related Product Information for GPT active protein

GPT, also known as alanine aminotransferases (ALT1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. This protein plays a key role in the intermediary metabolism of glucose and amino acids. It is widely used as an index of liver integrity or hepatocellular damage in clinical settings. Recombinant human GPT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Product Categories/Family for GPT active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 4885351
• NCBI GeneID: 2875
• NCBI Accession #: NP_005300.1
• NCBI GenBank Nucleotide #: NM_005309.2
• UniProt Accession #: P24298; P78398; Q93076; B0YJ18; D3DWM7
• Molecular Weight: 56.8 kDa (516 aa, 1-496 aa + His Tag)
• NCBI Official Full Name: alanine aminotransferase 1
• NCBI Official Synonym Full Names: glutamic-pyruvate transaminase (alanine aminotransferase)
• NCBI Official Symbol: GPT
• NCBI Official Synonym Symbols: AAT1; ALT1; GPT1
• NCBI Protein Information: alanine aminotransferase 1
• UniProt Protein Name: Alanine aminotransferase 1
• Protein Family: Alanine aminotransferase
• UniProt Gene Name: GPT
• UniProt Synonym Gene Names: AAT1; GPT1; ALT1; GPT 1
• UniProt Entry Name: ALAT1_HUMAN

NCBI Description

This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009]

Uniprot Description

GPT: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

Protein type: Transferase; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.2

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cytosol

Molecular Function: alanine transaminase activity; pyridoxal phosphate binding

Biological Process: amino acid biosynthetic process; L-alanine catabolic process