GPT2/ALT2 Active Protein | GPT2 active protein

GPT2 / ALT2, human recombinant

Cat. Num. AAA844578

• Gene Names: GPT2; ALT2; GPT 2; MRT49
• Applications: SDS-Page
• Purity: >=90%
• Synonyms: GPT2/ALT2; GPT2 / ALT2; human recombinant; Alanine aminotransferase 2; AAT2; ALT2; GPT2 active protein

• Host: E Coli
• Purity/Purification: >=90%
• Form/Format: 0.5 mg/ml solution 20 mM Tris-HCl buffer (pH 7.5), 30% glycerol, 0.2 M NaCl and 2 mM DTT.; Appearance: Liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMQRAAAL VRRGCGPRTP SSWGRSQSSA AAEASAVLKV RPERSRRERI LTLESMNPQV KAVEYAVRGP IVLKAGEIEL ELQRGIKKPF TEVIRANIGD AQAMGQQPIT FLRQVMALCT YPNLLDSPSF PEDAKKRARR ILQACGGNSL GSYSASQGVN CIREDVAAYI TRRDGGVPAD PDNIYLTTGA SDGISTILKI LVSGGGKSRT GVMIPIPQYP LYSAVISELD AIQVNYYLDE ENCWALNVNE LRRAVQEAKD HCDPKVLCII NPGNPTGQVQ SRKCIEDVIH FAWEEKLFLL ADEVYQDNVY SPDCRFHSFK KVLYEMGPEY SSNVELASFH STSKGYMGEC GYRGGYMEVI NLHPEIKGQL VKLLSVRLCP PVSGQAAMDI VVNPPVAGEE SFEQFSREKE SVLGNLAKKA KLTEDLFNQV PGIHCNPLQG AMYAFPRIFI PAKAVEAAQA HQMAPDMFYC MKLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPVEKLKTV LQKVKDFHIN FLEKYA
• Sequence Length: 423

• Applicable Applications for GPT2 active protein: SDS-PAGE
• Biological Activity: Specific activity is > 30 unit/mg; One unit will convert 1.0 umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37 degree C.
• Results: Specific activity is > 30 unit/mg
• Unit Definition: One unit will convert 1.0 umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37 degree C.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for GPT2 active protein

Background: Alanine aminotransferase 2, also known as GPT2, catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Subsequently, they play a key role in the intermediary metabolism of glucose and amino acids. GPT2 expression is high in muscle, fat and kidney. Recombinant human GPT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Product Categories/Family for GPT2 active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 215599424
• NCBI GeneID: 84706
• NCBI Accession #: NP_001135938.1
• NCBI GenBank Nucleotide #: NM_001142466.2
• UniProt Accession #: Q8TD30; Q8N9E2
• Molecular Weight: 60.3 kDa (546 aa, 1-523 aa + His Tag)
• NCBI Official Full Name: alanine aminotransferase 2 isoform 2
• NCBI Official Synonym Full Names: glutamic pyruvate transaminase (alanine aminotransferase) 2
• NCBI Official Symbol: GPT2
• NCBI Official Synonym Symbols: ALT2; GPT 2; MRT49
• NCBI Protein Information: alanine aminotransferase 2
• UniProt Protein Name: Alanine aminotransferase 2
• Protein Family: Glycerol-3-phosphate O-acyltransferase
• UniProt Gene Name: GPT2
• UniProt Synonym Gene Names: AAT2; ALT2; ALT2; GPT 2
• UniProt Entry Name: ALAT2_HUMAN

NCBI Description

This gene encodes a mitochondrial alanine transaminase, a pyridoxal enzyme that catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate. Alanine transaminases play roles in gluconeogenesis and amino acid metabolism in many tissues including skeletal muscle, kidney, and liver. Activating transcription factor 4 upregulates this gene under metabolic stress conditions in hepatocyte cell lines. A loss of function mutation in this gene has been associated with developmental encephalopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

Uniprot Description

GPT2: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.2; Transferase; Mitochondrial

Chromosomal Location of Human Ortholog: 16q12.1

Cellular Component: mitochondrial matrix

Molecular Function: alanine transaminase activity; pyridoxal phosphate binding

Biological Process: amino acid biosynthetic process; L-alanine metabolic process; L-alanine catabolic process; 2-oxoglutarate metabolic process

Disease: Mental Retardation, Autosomal Recessive 49