FGF-2/FGF-basic Active Protein | FGF-2 active protein

FGF-2 / FGF-basic, human recombinant

Cat. Num. AAA844920

• Gene Names: FGF2; BFGF; FGFB; FGF-2; HBGF-2
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: FGF-2/FGF-basic; FGF-2 / FGF-basic; human recombinant; FGF2; HBGF-2; Prostatropin; Basic fibroblast growth factor; Heparin-binding growth factor 2; HBGH-2; FGF-2; FGF-b; Fibroblast Growth Factor; FGF-2 active protein

• Host: E Coli
• Purity/Purification: >=95%
• Form/Format: FGF-2 protein is lyophilized from a 1 mg/ml solution of 5 mM Tris pH 7.6 + 150 mM NaCl; Appearance: Recombinant FGF-2 is available as a lyophilized powder
• Sequence Length: 288

• Applicable Applications for FGF-2 active protein: SDS-PAGE; HPLC
• Solubility/Reconstitution Instructions: Reconstitute human FGF-2 in 5 mM Tris, pH 7.6 to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers
• Endotoxin Level: <0.1 ng/mug
• Biological Activity: The ED?? of human FGF-2 is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells and is typically less than 3 ng/ml.
• Results: <0.5 ng/ml
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for FGF-2 active protein

Background: FGF-2 (FGF2, FGF 2, FGF-basic, basic FGF, bFGF) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-2 protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different FGF-2 isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.

Product Categories/Family for FGF-2 active protein

Proteins and Enzymes; Cytokines; Growth Factors & Hormones; FGFsProteins and Enzymes; Cytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Proteins and Enzymes (A-Z) Proteins and Enzymes; Animal-Free Origin (AOF)

NCBI and Uniprot Product Information

• NCBI GI #: 153285461
• NCBI GeneID: 2247
• NCBI Accession #: NP_001997.5
• NCBI GenBank Nucleotide #: NM_002006.4
• UniProt Accession #: P09038; O00527; P78443; Q16443; Q5PY50; Q7KZ11; Q7KZ72; Q9UC54; Q9UCS5; Q9UCS6; A4LBB8
• Molecular Weight: Human FGF-2 is a 17.2 kDa recombinant protein
• NCBI Official Full Name: fibroblast growth factor 2
• NCBI Official Synonym Full Names: fibroblast growth factor 2 (basic)
• NCBI Official Symbol: FGF2
• NCBI Official Synonym Symbols: BFGF; FGFB; FGF-2; HBGF-2
• NCBI Protein Information: fibroblast growth factor 2
• UniProt Protein Name: Fibroblast growth factor 2
• UniProt Gene Name: FGF2
• UniProt Synonym Gene Names: FGFB; FGF-2; bFGF; HBGF-2
• UniProt Entry Name: FGF2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF2: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2. Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue. Belongs to the heparin-binding growth factors family. 4 isoforms of the human protein are produced by alternative initiation.

Protein type: Activator; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q26

Cellular Component: extracellular space; extracellular region; nucleus

Molecular Function: heparin binding; protein binding; ligand-dependent nuclear receptor transcription coactivator activity; growth factor activity; cytokine activity; chemoattractant activity; fibroblast growth factor receptor binding

Biological Process: extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; wound healing; activation of MAPK activity; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; chemotaxis; signal transduction; hyaluronan catabolic process; growth factor dependent regulation of satellite cell proliferation; positive regulation of MAP kinase activity; positive chemotaxis; positive regulation of cell proliferation; embryonic morphogenesis; positive regulation of cardiac muscle cell proliferation; epidermal growth factor receptor signaling pathway; nervous system development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; positive regulation of cell fate specification; positive regulation of blood vessel endothelial cell migration; positive regulation of phosphoinositide 3-kinase activity; negative regulation of blood vessel endothelial cell migration; regulation of angiogenesis; positive regulation of angiogenesis; organ morphogenesis; cell migration during sprouting angiogenesis; ureteric bud branching; release of sequestered calcium ion into cytosol; positive regulation of cell division; Ras protein signal transduction; phosphatidylinositol biosynthetic process; insulin receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; inositol phosphate biosynthetic process