Western Blot (WB)
(Western blot analysis of MARCH5 Antibody (N-term) in mouse kidney tissue lysates (35ug/lane).MARCH5 (arrow) was detected using the purified Pab.)
Rabbit MARCH5 Polyclonal Antibody | anti-MARCH5 antibody
MARCH5 Antibody (N-term)
Western Blot (WB)
(Western blot analysis of MARCH5 Antibody (N-term) in mouse kidney tissue lysates (35ug/lane).MARCH5 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of MARCH5 Antibody (N-term) in mouse kidney tissue lysates (35ug/lane).MARCH5 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(MARCH5 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MARCH5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(MARCH5 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MARCH5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(MARCH5 Antibody (N-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(MARCH5 Antibody (N-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).[supplied by OMIM, Mar 2008]
Uniprot Description
MARCH5: Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission. May play a role in the prevention of cell senescence acting as a regulator of mitochondrial quality control. Promotes ubiquitination of FIS1, DNM1L and MFN1.
Protein type: EC 6.3.2.19; Membrane protein, integral; EC 6.3.2.-; Membrane protein, multi-pass; Ubiquitin ligase; Ubiquitin conjugating system; Ligase
Chromosomal Location of Human Ortholog: 10q23.32-q23.33
Cellular Component: mitochondrial outer membrane; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane
Molecular Function: GTPase binding; protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity
Biological Process: protein autoubiquitination; protein polyubiquitination
Research Articles on MARCH5
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Product Notes
The MARCH5 march5 (Catalog #AAA9208208) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 4-32. The MARCH5 Antibody (N-term) reacts with Human, mouse (Predicted Reactivity: Bovine, Chicken, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's MARCH5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the MARCH5 march5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MARCH5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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