AKR1B1 recombinant protein

AKR1B1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120776

• Gene Names: AKR1B1; AR; ADR; ALR2; ALDR1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: AKR1B1; AKR1B1 Protein; Human; Recombinant (His Tag); Human AKR1B1 Protein (His Tag); ADR Protein; ALDR1 Protein; ALR2 Protein; AR Protein; MGC1804 Protein; aldo-keto reductase family 1; member B1 (aldose reductase); AKR1B1 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 20% glycerol, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Phe316

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human AKR1B1 (P15121)(Met 1-Phe 316) was expressed, with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for AKR1B1 recombinant protein

Background: Aldose reductase (AKR1B1) belongs to the aldo/keto reductase superfamily. AKR1B1 is a NADPH-dependent aldo-keto reductase best known as the rate-limiting enzyme of the polyol pathway. Expression of AKR1B1 was the highest in lens and retina. It is the first enzyme in the polyol pathway through which glucose is converted to sorbitol which is important for the function of various organs in the body, and has been implicated in the etiology of diabetic complications. AKR1B1 is quite abundant in the collecting tubule cells and thought to provide protection against hypertonic environment. Some human tissues contain AKR1B1 as well as AKR1B10, a closely related member of the aldo-keto reductase superfamily.

References

Huang SP, et al. (2010) Aldo-Keto Reductases in the Eye. Journal of Ophthalmology. 326 (3): 625-36.Aida K, et al. (2000) Disruption of Aldose Reductase Gene (Akr1b1) Causes Defect in Urinary Concentrating Ability and Divalent Cation Homeostasis. Biochemical and Biophysical Research Communications.277 (2): 281-6.Liao CS, et al. (2009) Regulation of AKR1B1 by thyroid hormone and its receptors. Molecular and Cellular Endocrinology. 307 (1-2): 109-17.Baba SP, et al. (2009) Posttranslational glutathiolation of aldose reductase (AKR1B1): A possible mechanism of protein recovery from S-nitrosylation. Chemico-Biological Interactions. 178 (1-3): 250-8.

NCBI and Uniprot Product Information

• NCBI GI #: 4502049
• NCBI GeneID: 231
• NCBI Accession #: NP_001619.1
• NCBI GenBank Nucleotide #: NM_001628.2
• UniProt Accession #: P15121; Q5U031; Q6FGA4; Q6ICP2; Q9BS21; Q9UCI9; B2R8N3
• Molecular Weight: 35,853 Da
• NCBI Official Full Name: aldose reductase
• NCBI Official Synonym Full Names: aldo-keto reductase family 1, member B1 (aldose reductase)
• NCBI Official Symbol: AKR1B1
• NCBI Official Synonym Symbols: AR; ADR; ALR2; ALDR1
• NCBI Protein Information: aldose reductase; aldehyde reductase 1; low Km aldose reductase; Lii5-2 CTCL tumor antigen; aldo-keto reductase family 1 member B1
• UniProt Protein Name: Aldose reductase
• Protein Family: Aldose reductase
• UniProt Gene Name: AKR1B1
• UniProt Synonym Gene Names: ALDR1; AR
• UniProt Entry Name: ALDR_HUMAN

NCBI Description

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq, Feb 2009]

Uniprot Description

AKR1B1: Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. Belongs to the aldo/keto reductase family.

Protein type: Carbohydrate Metabolism - pentose and glucuronate interconversions; Oxidoreductase; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - galactose; EC 1.1.1.21; Lipid Metabolism - glycerolipid; Carbohydrate Metabolism - fructose and mannose

Chromosomal Location of Human Ortholog: 7q35

Cellular Component: nucleoplasm; extracellular space; cytoplasm; cytosol

Molecular Function: glyceraldehyde oxidoreductase activity; electron carrier activity; aldo-keto reductase activity; aldehyde reductase activity

Biological Process: steroid metabolic process; carbohydrate metabolic process; C21-steroid hormone biosynthetic process; response to stress