Rabbit Glycogen Synthase Kinase 3 beta, aa335-349 Polyclonal Antibody | anti-GSK3B antibody

Glycogen Synthase Kinase 3 beta, aa335-349 (GSK-3 Beta)

Cat. Num. AAA6004469

• Reactivity: Bovine, Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Glycogen Synthase Kinase 3 beta; aa335-349; Polyclonal Antibody; aa335-349 (GSK-3 Beta); Anti -Glycogen Synthase Kinase 3 beta; anti-GSK3B antibody

• Host: Rabbit
• Reactivity: Bovine, Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes GSK3 beta. Species Crossreactivity: rat, mouse, human, bovine.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 50% glycerol, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-GSK3B antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.; Dilution: Western Blot: 1:500; Immunohistochemistry (paraffin): 1ug/ml; Immunoprecipitation: 12.5ug/ml
• Immunogen: Rat GSK3 beta synthetic peptide (aa335-349), conjugated to KLH. This sequence is 100% identical to human GSK3 beta.
• Positive Control: C2C12 Cell Lysate, Hela cells, rat or mouse brain
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Immunohistochemistry(paraffin): Representative images from a previous lot. Optimal Staining of GSK3β Polyclonal Antibody: HeLa Cells GSK3β staining on HeLa cells, pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was used at 1ug/mL, using IHC-Select® detection with HRP-DAB. Immunoreactivity is detected in tumor cells.)

Western Blot (WB)

(Western Blot Analysis: C2C12 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-GSK3 BETA (1:500 dilution of this lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein GSK3 BETA (~47kD).)

Western Blot (WB)

(Western Blot Analysis: Representative image from a previous lot. RAW 264 lysate (Lane1), NIH/3T3 lysate (Lane 2), C2C12 lysate (Lane 3) and PC12 lysate (Lane 4) were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-GSK3 BETA (1:500 dilution of a previous lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein GSK3 BETA (~47kD).)

Related Product Information for anti-GSK3B antibody

GSK3 (Glycogen Synthase Kinase 3) differs from most serine/threonine kinases in that it is active in the absence of the action of signaling pathways. Two isoforms exist, GSK3 and GSK3. The function of GSK3 is to phosphorylate Glycogen Synthase and thereby inactivate it. Insulin action stimulates the PI3 Kinase pathway, resulting in Akt activation, which phosphorylates and inactivates GSK3. Glycogen Synthase is then rapidly dephosphorylated and activated. Other GSK3 substrates include Jun (on inhibitory sites), and eIF2B. Phosphorylation of Tau by GSK3 may relate to development of Alzheimer's disease. Phosphorylation state-specific antibodies directed against the Akt site (Ser21) on GSK3 are suitable for surrogate assays of the activation state of the pathway.

Product Categories/Family for anti-GSK3B antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 5730335
• NCBI GeneID: 2932
• UniProt Accession #: P49841; Q9BWH3; Q9UL47; D3DN89
• Molecular Weight: 46,744 Da
• NCBI Official Full Name: glycogen synthase kinase 3 beta
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 beta
• NCBI Official Symbol: GSK3B
• NCBI Protein Information: glycogen synthase kinase-3 beta; GSK-3 beta; GSK3beta isoform; serine/threonine-protein kinase GSK3B
• UniProt Protein Name: Glycogen synthase kinase-3 beta
• Protein Family: Glycogen synthase kinase
• UniProt Gene Name: GSK3B
• UniProt Synonym Gene Names: GSK-3 beta
• UniProt Entry Name: GSK3B_HUMAN

NCBI Description

The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

GSK3B: a proline-directed protein kinase of the GSK family. Phosphorylates and inactivates glycogen synthase. Participates in the Wnt signaling pathway. Involved in energy metabolism, neuronal cell development, and body pattern formation

Protein type: Protein kinase, CMGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; EC 2.7.11.26; CMGC group; GSK family; GSK subfamily

Chromosomal Location of Human Ortholog: 3q13.3

Cellular Component: centrosome; dendritic spine; beta-catenin destruction complex; cytosol; ribonucleoprotein complex; lipid raft; nucleoplasm; growth cone; cell soma; perinuclear region of cytoplasm; cytoplasm; plasma membrane; dendritic shaft; nucleus

Molecular Function: integrin binding; NF-kappaB binding; ionotropic glutamate receptor binding; protein serine/threonine kinase activity; protein binding; p53 binding; ubiquitin protein ligase binding; tau-protein kinase activity; beta-catenin binding; kinase activity; protein kinase binding; tau protein binding; ATP binding

Biological Process: circadian rhythm; glycogen metabolic process; fat cell differentiation; negative regulation of MAP kinase activity; axon guidance; re-entry into mitotic cell cycle; genetic imprinting; nerve growth factor receptor signaling pathway; positive regulation of protein binding; response to lithium ion; positive regulation of axon extension; protein amino acid autophosphorylation; Wnt receptor signaling pathway through beta-catenin; protein amino acid phosphorylation; positive regulation of protein export from nucleus; protein export from nucleus; positive regulation of cell-matrix adhesion; negative regulation of protein binding; myoblast fusion; ER overload response; negative regulation of glycogen biosynthetic process; epidermal growth factor receptor signaling pathway; response to drug; regulation of microtubule-based process; cell migration; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; hippocampus development; negative regulation of dendrite morphogenesis; negative regulation of NFAT protein import into nucleus; positive regulation of peptidyl-serine phosphorylation; organ morphogenesis; peptidyl-serine phosphorylation; negative regulation of neuron maturation; positive regulation of protein complex assembly; establishment of cell polarity; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein catabolic process; innate immune response; epithelial to mesenchymal transition; positive regulation of transcription from RNA polymerase II promoter; negative regulation of protein complex assembly; regulation of neuronal synaptic plasticity; negative regulation of apoptosis