FGF-19 active protein

Human FGF-19

Cat. Num. AAA695996

• Purity: >=95% as determined by SDS-PAGE gel and HPLC Analysis.
• Synonyms: FGF-19; Human FGF-19; Recombinant Human FGF-19; FGF-19 active protein

• Host: E Coli
• Purity/Purification: >=95% as determined by SDS-PAGE gel and HPLC Analysis.
• Form/Format: Sterile filtered through a 0.2 micron filter. Lyophilized 5mM Sodium Phosphate, pH 7.2 + 75 mM NaCI.
• Sequence: MRPLAFSDA GPHVHYGWG DPIRLRHLY TSGPHGLSS CFLRIRADG VVDCARGQS AHSLLEIKA VALRTVAIK GVHSVRYLC MGADGKMQG LLQYSEEDC AFEEEIRPD GYNVYRSEK HRLPVSLSS AKQRQLYKN RGFLPLSHF LPMLPMVPE EPEDLRGHL ESDMFSSPL ETDSMDPFG LVTGLEAVR SPSFEK

• Autheticity: Verified by N-terminal and Mass Spectometry analyses (when applicable)
• Endotoxin: Endotoxin level is <0.1 ng/ug of protein (<1EU/ug).
• Protein Content: Verified by V Spectroscopy and/or SDS-PAGE gel.
• Biological Activity: Determined by a cell proliferation assay using balb/c 3T3 cells. The expected ED50 for this effect is 100-150 ng/ml.
• Reconsitution: Centrifuge the vial prior to opening. Reconstitute in 5-10mM Sodium Phosphate,pH 8.0 to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
• Preparation and Storage: Store at -20°C

Testing Data

Related Product Information for FGF-19 active protein

The FGF family plays central roles during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-19, a member of the FGF family, is a high affinity heparin dependent ligand for FGFR4. FGF-19 is expressed during the brain development and embryogenesis. Recombinant human FGF-19 is a 21.8 kDa protein containing 195 amino acid residues.

Product Categories/Family for FGF-19 active protein

Stem Cell Research; Angiogenesis; Bone Biology; Proliferation and Cell Cycle

NCBI and Uniprot Product Information

• NCBI GI #: 11132196
• NCBI GeneID: 9965
• UniProt Accession #: O95750
• Molecular Weight: 24,003 Da
• NCBI Official Full Name: Fibroblast growth factor 19
• NCBI Official Synonym Full Names: fibroblast growth factor 19
• NCBI Official Symbol: FGF19
• NCBI Protein Information: fibroblast growth factor 19; FGF-19
• UniProt Protein Name: Fibroblast growth factor 19
• UniProt Gene Name: FGF19
• UniProt Synonym Gene Names: FGF-19
• UniProt Entry Name: FGF19_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion. This growth factor is a high affinity, heparin dependent ligand for FGFR4. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF19: Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4. Belongs to the heparin-binding growth factors family.

Protein type: Cell development/differentiation; Cytokine; Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: extracellular region

Molecular Function: protein binding; growth factor activity; fibroblast growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; nervous system development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; heart development; positive regulation of JNK cascade; positive regulation of glucose import; positive regulation of cell proliferation; insulin receptor signaling pathway; innate immune response; positive regulation of protein amino acid phosphorylation; neural crest cell migration