Rabbit anti-Human Nerve Growth Factor, beta Polyclonal Antibody | anti-NGF antibody

Nerve Growth Factor, beta (NGF, Beta Nerve Growth Factor, Beta Nerve Growth Factor Precursor, Beta NGF, HSAN5, Nerve Growth Factor beta, Nerve Growth Factor beta Polypeptide, Nerve Growth Factor beta Subunit, NGFB)

Cat. Num. AAA626076

• Gene Names: NGF; NGFB; HSAN5; Beta-NGF; MGC161426; MGC161428
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: Nerve Growth Factor; beta; Polyclonal Antibody; beta (NGF; Beta Nerve Growth Factor; Beta Nerve Growth Factor Precursor; Beta NGF; HSAN5; Nerve Growth Factor beta; Nerve Growth Factor beta Polypeptide; Nerve Growth Factor beta Subunit; NGFB); Anti -Nerve Growth Factor; anti-NGF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NGFB.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-NGF antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100; Immunohistochemistry: 1:10-1:50
• Immunogen: Synthetic peptide selected from the Center region of human NGFB (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of NGFB Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). NGFB (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with NGFB antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(NGFB Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of NGFB Antibody (Center) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-NGF antibody

NGFB is a member of the NGF-beta family. It is a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in the gene encoding NGFB have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of the gene's expression is associated with allergic rhinitis.

Product Categories/Family for anti-NGF antibody

Antibodies; Abs to Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 189203
• NCBI GeneID: 4803
• UniProt Accession #: P01138; Q6FHA0; Q96P60; Q9P2Q8; Q9UKL8; A1A4E5
• Molecular Weight: 26,959 Da
• NCBI Official Full Name: nerve growth factor beta
• NCBI Official Synonym Full Names: nerve growth factor (beta polypeptide)
• NCBI Official Symbol: NGF
• NCBI Official Synonym Symbols: NGFB; HSAN5; Beta-NGF; MGC161426; MGC161428
• NCBI Protein Information: beta-nerve growth factor; OTTHUMP00000013653; nerve growth factor, beta subunit
• UniProt Protein Name: Beta-nerve growth factor
• Protein Family: Nerve growth factor
• UniProt Gene Name: NGF
• UniProt Synonym Gene Names: NGFB
• UniProt Entry Name: NGF_HUMAN

NCBI Description

This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq]

Uniprot Description

NGF: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Homodimer. Belongs to the NGF-beta family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: extracellular space; Golgi lumen; cytoplasmic membrane-bound vesicle; extracellular region; endosome

Molecular Function: metalloendopeptidase inhibitor activity; nerve growth factor receptor binding; protein binding; growth factor activity; receptor signaling protein activity

Biological Process: circadian rhythm; response to nicotine; response to peptide hormone stimulus; activation of MAPKK activity; nerve growth factor receptor signaling pathway; regulation of neuron differentiation; positive regulation of apoptosis; adult locomotory behavior; positive regulation of axon extension; response to glucocorticoid stimulus; regulation of neurotransmitter secretion; response to lipopolysaccharide; regulation of axonogenesis; sensory perception of pain; regulation of caspase activity; negative regulation of cell cycle; nerve growth factor processing; response to radiation; cell-cell signaling; small GTPase mediated signal transduction; negative regulation of neuron apoptosis; inflammatory response; response to electrical stimulus; response to drug; positive regulation of neuron maturation; phosphoinositide-mediated signaling; positive regulation of protein amino acid autophosphorylation; positive regulation of nerve growth factor receptor signaling pathway; regulation of release of sequestered calcium ion into cytosol; memory; peripheral nervous system development; neuron apoptosis; induction of apoptosis via death domain receptors; phospholipase C activation; response to mechanical stimulus; response to ozone; Ras protein signal transduction; positive regulation of transcription factor activity; positive regulation of axonogenesis; neurite morphogenesis; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis

Disease: Neuropathy, Hereditary Sensory And Autonomic, Type V