FGF-23 recombinant protein

Human FGF-23

Cat. Num. AAA696202

• Gene Names: FGF23; ADHR; FGFN; HYPF; HPDR2; PHPTC
• Reactivity: Species Reactivity: Human
• Purity: ≥ 95% as determined by SDS-PAGE gel and HPLC analyses.
• Synonyms: FGF-23; Human FGF-23; Recombinant Human FGF-23; Fibroblast Growth Factor-23; FGF-23 recombinant protein

• Host: E Coli
• Reactivity: Species Reactivity: Human
• Purity/Purification: ≥ 95% as determined by SDS-PAGE gel and HPLC analyses.
• Form/Format: Sterile Filtered through a 0.2 micron filter. Lyophilized from 10mM Sodium Acetate, pH 6.0 + 0.1 M Arginine.
• Sequence: MYPNASPLL GSSWGGLIH LYTATARNS YHLQIHKNG HVDGAPHQT IYSALMIRS EDAGFVVIT GVMSRRYLC MDFRGNIFG SHYFDPENC RFQHQTLEN GYDVYHSPQ YHFLVSLGR AKRAFLPGM NPPPYSQFL SRRNEIPLI HFNTPIPRR HTRSAEDDS ERDPLNVLK PRARMTPAP ASCSQELPS AEDNSPMAS DPLGVVRGG RVNTHAGGT GPEGCRPFA KFI

• Authenticity: Verified by N-terminal and Mass Spectrometry analyses.
• Endotoxin: Endotoxin level if <0.1 ng/ug of protein (<1EU/ug).
• Cell Type: Stem Cells
• Reconstitution: Centrifuge before opening. Reconstitute in water to a concentration of 0.5 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up one week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
• Preparation and Storage: At -20 degree C

Testing Data

Related Product Information for FGF-23 recombinant protein

The FGF family plays a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-23, FGF-21 and FGF-19 constitute an atypical FGF subfamily whose ligands act as circulating hormones and require the participation of a Klotho protein as a co-receptor for their signaling. FGF-23 is a bone-derived hormone that acts in the kidney to regulate phosphate homeostasis and vitamin D metabolism. The signaling receptor for FGF-23, a Klotho-FGFR1 (IIIc) complex, is an essential regulator of the renal sodium phosphate co-transporter and key vitamin D-metabolizing enzymes CYP27B1 and CYP24A1. Recombinant human FGF-23 is a 22.5 kDa globular protein containing 228 amino acid residues.

Product Categories/Family for FGF-23 recombinant protein

Stem Cell Research; Angiogenesis; Bone Biology; Proliferation and Cell Cycle; Metabolic Regulation and Diabetes

NCBI and Uniprot Product Information

• NCBI GI #: 13626688
• NCBI GeneID: 8074
• UniProt Accession #: Q9GZV9; Q4V758
• NCBI Official Full Name: Fibroblast growth factor 23
• NCBI Official Synonym Full Names: fibroblast growth factor 23
• NCBI Official Symbol: FGF23
• NCBI Official Synonym Symbols: ADHR; FGFN; HYPF; HPDR2; PHPTC
• NCBI Protein Information: fibroblast growth factor 23; phosphatonin; tumor-derived hypophosphatemia inducing factor
• UniProt Protein Name: Fibroblast growth factor 23
• UniProt Gene Name: FGF23
• UniProt Synonym Gene Names: HYPF; FGF-23
• UniProt Entry Name: FGF23_HUMAN

NCBI Description

This gene encodes a member of the fibroblast growth factor family of proteins, which possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. The product of this gene regulates phosphate homeostasis and transport in the kidney. The full-length, functional protein may be deactivated via cleavage into N-terminal and C-terminal chains. Mutation of this cleavage site causes autosomal dominant hypophosphatemic rickets (ADHR). Mutations in this gene are also associated with hyperphosphatemic familial tumoral calcinosis (HFTC). [provided by RefSeq, Feb 2013]

Uniprot Description

FGF23: Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Upregulates EGR1 expression in the presence of KL. Acts directly on the parathyroid to decrease PTH secretion. Regulator of vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization. Defects in FGF23 are the cause of autosomal dominant hypophosphataemic rickets (ADHR). ADHR is characterized by low serum phosphorus concentrations, rickets, osteomalacia, leg deformities, short stature, bone pain and dental abscesses. Defects in FGF23 are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC). HFTC is a severe autosomal recessive metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Belongs to the heparin-binding growth factors family.

Protein type: Secreted; Cytokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: extracellular space; extracellular region

Molecular Function: growth factor activity; type 1 fibroblast growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of bone mineralization; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; cellular phosphate ion homeostasis; nerve growth factor receptor signaling pathway; negative regulation of hormone secretion; positive regulation of transcription, DNA-dependent; insulin receptor signaling pathway; innate immune response; negative regulation of osteoblast differentiation; phosphate ion homeostasis; cell differentiation; phosphate metabolic process; vitamin D catabolic process

Disease: Hypophosphatemic Rickets, Autosomal Dominant