Rabbit anti-Human, Mouse POLR2H Polyclonal Antibody | anti-POLR2H antibody

POLR2H Polyclonal Antibody

Cat. Num. AAA3011858

• Gene Names: POLR2H; RPB8; RPB17; RPABC3
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: POLR2H; Polyclonal Antibody; POLR2H Polyclonal Antibody; DNA-directed RNA polymerases I; II; and III subunit RPABC3; RNA polymerases I; and III subunit; ABC3; DNA-directed RNA polymerase II subunit H; and III 17.1 kDa polypeptide; RPB17; RPB8 homolog; hRPB8; anti-POLR2H antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: POLR2H polyclonal antibody detects endogenous levels of POLR2H protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 150

• Applicable Applications for anti-POLR2H antibody: Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200; IP: 1:50-1:200
• Immunogen: Recombinant full length Human POLR2H.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using POLR2H antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U20S cell using POLR2H antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-POLR2H antibody

Eukaryotes produce 3 distinct classes of RNA polymerase, Pol I, II and III. Each polymerase is responsible for the synthesis of a different class of RNA. RNA polymerase I (Pol I) transcribes the rRNA (ribosomal RNA) genes for the precursor of the 28S, 18S, and 5.8S molecules of the ribosome. RNA polymerase II transcribes protein-encoding genes into mRNA (messenger RNA) and snRNA (small nuclear RNA) genes into snRNAs that influence the processing of other classes of RNA. RNA polymerase III (Pol III) transcribes the 5S rRNA genes and all of the tRNA (transfer RNA) genes. Each class of RNA polymerase is assembled from 9 to 15 different polypeptides. The RPB6 and RPB8 subunits are shared by all 3 RNA polymerases.

NCBI and Uniprot Product Information

• NCBI GI #: 14589953
• NCBI GeneID: 5437
• NCBI Accession #: NP_006223.2
• NCBI GenBank Nucleotide #: NP_006223.2
• UniProt Accession #: P52434
• Molecular Weight: ~ 17kDa
• NCBI Official Full Name: DNA-directed RNA polymerases I, II, and III subunit RPABC3 isoform 2
• NCBI Official Synonym Full Names: RNA polymerase II subunit H
• NCBI Official Symbol: POLR2H
• NCBI Official Synonym Symbols: RPB8; RPB17; RPABC3
• NCBI Protein Information: DNA-directed RNA polymerases I, II, and III subunit RPABC3
• UniProt Protein Name: DNA-directed RNA polymerases I, II, and III subunit RPABC3
• UniProt Gene Name: POLR2H
• UniProt Synonym Gene Names: RNA polymerases I, II, and III subunit ABC3; hRPB8
• UniProt Entry Name: RPAB3_HUMAN

NCBI Description

The three eukaryotic RNA polymerases are complex multisubunit enzymes that play a central role in the transcription of nuclear genes. This gene encodes an essential and highly conserved subunit of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]