Horse PAI1 (Plasminogen activator inhibitor 1) ELISA Kit | PAI1 elisa kit

Horse PAI1 (Plasminogen activator inhibitor 1)

Cat. Num. AAA766166

• Gene Names: SERPINE1; PAI; PAI1; PAI-1; PLANH1
• Reactivity: Horse
• Synonyms: PAI1 (Plasminogen activator inhibitor 1); Horse PAI1 (Plasminogen activator inhibitor 1); PAI-1/PAI1/SERPINE1/Nexin/PLANH1/Endothelial plasminogen activator inhibitor/PAISerpin E1/PLANH1/plasminogen activator inhibitor 1/serine (or cysteine) proteinase inhibitor; clade E (nexin; plasminogenactivator inhibitor type 1); member 1/serpin peptidase inhibitor; plasminogen activator inhibitortype 1); member 1; PAI1 elisa kit

• Reactivity: Horse
• Specificity: This assay has high sensitivity and excellent specificity for detection of PAI1 . No significant cross-reactivity or interference between PAI1 and analogues was observed.
• Sequence Length: 402

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Competitive ELISA, Coated with Antigen
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PAI1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PAI1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: 4 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PAI1 elisa kit

Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with PAI1 . During the reaction,PAI1 in the sample or standard competes with a fixed amount of PAI1 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to PAI1 . Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PAI1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 10835159
• NCBI GeneID: 5054
• NCBI Accession #: NP_000593.1
• NCBI GenBank Nucleotide #: NM_000602.4
• Molecular Weight: 43,404 Da
• NCBI Official Full Name: plasminogen activator inhibitor 1
• NCBI Official Synonym Full Names: serpin family E member 1
• NCBI Official Symbol: SERPINE1
• NCBI Official Synonym Symbols: PAI; PAI1; PAI-1; PLANH1
• NCBI Protein Information: plasminogen activator inhibitor 1
• UniProt Protein Name: Plasminogen activator inhibitor 1
• UniProt Gene Name: SERPINE1
• UniProt Synonym Gene Names: PAI1; PLANH1; PAI; PAI-1
• UniProt Entry Name: PAI1_HUMAN

NCBI Description

This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

SERPINE1: a secreted protein that acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Belongs to the serpin family. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Plasma levels of PAI-1 and VCAM-1 together may be useful in predicting post-operative recurrence in patients with colorectal cancer.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: extracellular matrix; extracellular region; extracellular space; plasma membrane

Molecular Function: protease binding; protein binding; receptor binding; serine-type endopeptidase inhibitor activity

Biological Process: angiogenesis; chronological cell aging; circadian rhythm; defense response to Gram-negative bacterium; extracellular matrix organization and biogenesis; fibrinolysis; negative regulation of blood coagulation; negative regulation of cell adhesion mediated by integrin; negative regulation of cell migration; negative regulation of fibrinolysis; negative regulation of smooth muscle cell migration; platelet degranulation; positive regulation of angiogenesis; positive regulation of blood coagulation; positive regulation of inflammatory response; positive regulation of interleukin-8 production; positive regulation of receptor-mediated endocytosis; positive regulation of transcription from RNA polymerase II promoter; regulation of receptor activity

Disease: Plasminogen Activator Inhibitor-1 Deficiency