Western Blot (WB)
(Western blot analysis of extracts from K-562 cells, using NOP58 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit NOP58 Polyclonal Antibody | anti-NOP58 antibody
NOP58 Antibody
Gene Names
NOP58; NOP5; HSPC120; NOP5/NOP58
Reactivity
Human, Mouse, RatPredicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
NOP58; Polyclonal Antibody; NOP58 Antibody; HSPC120; NOL5; NOP5; NOP5/NOP58; NOP58 ribonucleoprotein homolog (yeast); NOP58 ribonucleoprotein homolog; NOP58_HUMAN; Nucleolar protein 5; Nucleolar protein 58; Nucleolar protein NOP5/NOP58; anti-NOP58 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Clonality
Polyclonal
Isotype
IgG
Specificity
NOP58 Antibody detects endogenous levels of NOP58.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
529
Applicable Applications for anti-NOP58 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human NOP58.
Tissue Specificity
Ubiquitous.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from K-562 cells, using NOP58 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from K-562 cells, using NOP58 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-NOP58 antibody
Description: Required for 60S ribosomal subunit biogenesis (By similarity). Core component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles. Required for the biogenesis of box C/D snoRNAs such as U3, U8 and U14 snoRNAs.
Post Translational Modifications: Sumoylation is essential for high-affinity binding to snoRNAs.
Subunit Structure: Core component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles; the core proteins SNU13, NOP56, NOP58 and FBL assemble stepwise onto the snoRNA. Interacts with NOLC1/Nopp140. Interacts with NUFIP1, RUVBL1 AND RUVBL2; RUVBL1:RUVBL2 seem to bridge the association of NOP58 with NUFIP1. Interacts with PIH1D1 (PubMed:17636026).
Similarity: Belongs to the NOP5/NOP56 family.
Post Translational Modifications: Sumoylation is essential for high-affinity binding to snoRNAs.
Subunit Structure: Core component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles; the core proteins SNU13, NOP56, NOP58 and FBL assemble stepwise onto the snoRNA. Interacts with NOLC1/Nopp140. Interacts with NUFIP1, RUVBL1 AND RUVBL2; RUVBL1:RUVBL2 seem to bridge the association of NOP58 with NUFIP1. Interacts with PIH1D1 (PubMed:17636026).
Similarity: Belongs to the NOP5/NOP56 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed: 60 kDa
Predicted: 60 kDa
Predicted: 60 kDa
NCBI Official Full Name
Nucleolar protein 58
NCBI Official Synonym Full Names
NOP58 ribonucleoprotein
NCBI Official Symbol
NOP58
NCBI Official Synonym Symbols
NOP5; HSPC120; NOP5/NOP58
NCBI Protein Information
nucleolar protein 58
UniProt Protein Name
Nucleolar protein 58
Protein Family
UniProt Gene Name
NOP58
UniProt Synonym Gene Names
NOL5; NOP5
UniProt Entry Name
NOP58_HUMAN
NCBI Description
The protein encoded by this gene is a core component of box C/D small nucleolar ribonucleoproteins. Some box C/D small nucleolar RNAs (snoRNAs), such as U3, U8, and U14, are dependent upon the encoded protein for their synthesis. This protein is SUMOylated, which is necessary for high affinity binding to snoRNAs. [provided by RefSeq, Nov 2015]
Uniprot Description
NOP5: Required for 60S ribosomal subunit biogenesis. Belongs to the NOP5/NOP56 family.
Protein type: Nucleolus; Cell development/differentiation; RNA-binding
Chromosomal Location of Human Ortholog: 2q33.1
Cellular Component: Cajal body; small nucleolar ribonucleoprotein complex; membrane; cytoplasm; nucleolus; nucleus
Molecular Function: snoRNA binding; protein binding
Biological Process: rRNA modification; snRNP protein import into nucleus; cell growth; rRNA processing
Research Articles on NOP58
Similar Products
Product Notes
The NOP58 nop58 (Catalog #AAA9611942) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NOP58 Antibody reacts with Human, Mouse, Rat Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's NOP58 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the NOP58 nop58 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NOP58, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
