CXCL4 (PF4) Active Protein | CXCL4 active protein

Recombinant Mouse CXCL4 (PF4)

Cat. Num. AAA4159946

• Gene Names: Pf4; Cxcl4; Scyb4
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CXCL4 (PF4); Recombinant Mouse CXCL4 (PF4); C-X-C motif chemokine 4; PF-4; Recombinant Mouse Platelet Factor 4 (PF4); CXCL4 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from a 0.2 um filtered solution in PB and 1.5M NaCl pH 7.4.
• Sequence Length: 105

• Bioactivity: Determined by its ability to inhibit human FGF-basic-dependent proliferation of NR6R-3T3 mouse fibroblasts. The ED50 for this effect is typically 5.0-15 ug/mL.
• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method.
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Related Product Information for CXCL4 active protein

Platelet factor 4 (PF4/CXCL4) is an ELRˆ' tetrameric, cationic chemokine that constitutes 25% of the protein in platelet alpha-granules. It is also found bound to the luminal vascular endothelial surface. Although platelets represent the primary source of PF4, it is also expressed at lower levels in other cells of the immune system including cultured T cells, monocytes, and endothelial and smooth muscle cells.

NCBI and Uniprot Product Information

• NCBI GI #: 9910486
• NCBI GeneID: 56744
• NCBI Accession #: NP_064316.1
• NCBI GenBank Nucleotide #: NP_064316.1
• UniProt Accession #: Q9Z126
• Molecular Weight: 9.0 kDa
• NCBI Official Full Name: platelet factor 4
• NCBI Official Synonym Full Names: platelet factor 4
• NCBI Official Symbol: Pf4
• NCBI Official Synonym Symbols: Cxcl4; Scyb4
• NCBI Protein Information: platelet factor 4
• UniProt Protein Name: Platelet factor 4
• UniProt Gene Name: Pf4
• UniProt Synonym Gene Names: Cxcl4; Scyb4; PF-4
• UniProt Entry Name: PLF4_MOUSE

Uniprot Description

PF4: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Motility/polarity/chemotaxis; Chemokine; Apoptosis; Secreted, signal peptide; Secreted

Cellular Component: extracellular space; protein complex; extracellular region; vesicle

Molecular Function: heparin binding; CXCR3 chemokine receptor binding; chemokine activity; CXCR chemokine receptor binding; cytokine activity

Biological Process: negative regulation of cytolysis; platelet activation; cytokine and chemokine mediated signaling pathway; leukocyte chemotaxis; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; positive regulation of cAMP metabolic process; positive regulation of tumor necrosis factor production; chemotaxis; negative regulation of megakaryocyte differentiation; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; negative regulation of MHC class II biosynthetic process; immune response; protein complex assembly; positive regulation of transcription from RNA polymerase II promoter; inflammatory response; positive regulation of macrophage differentiation