Human CXCL4 ELISA Kit | PF4 elisa kit

Human CXCL4 ELISA Kit

Cat. Num. AAA8248386

• Gene Names: PF4; PF-4; CXCL4; SCYB4
• Reactivity: Human
• Synonyms: CXCL4; Human CXCL4 ELISA Kit; SCYB4; Platelet factor 4; PF-4; C-X-C motif chemokine 4; Iroplact; Oncostatin-A; PF4 elisa kit

• Reactivity: Human
• Specificity: The Human CXCL4 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CXCL4 proteins within the range of 156 pg/ml - 10000 pg/ml.
• Sequence Length: 101

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <15 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PF4 elisa kit

Principle of the Assay: The Human CXCL4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CXCL4 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human CXCL4 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CXCL4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). The PF4 gene was localized on 4q12-q13. Chemokines play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.

NCBI and Uniprot Product Information

• NCBI GI #: 4505733
• NCBI GeneID: 5196
• NCBI Accession #: NP_002610.1
• NCBI GenBank Nucleotide #: NM_002619.3
• UniProt Accession #: P02776; Q53X61; Q9UC64; Q9UC65
• Molecular Weight: 10,845 Da
• NCBI Official Full Name: platelet factor 4
• NCBI Official Synonym Full Names: platelet factor 4
• NCBI Official Symbol: PF4
• NCBI Official Synonym Symbols: PF-4; CXCL4; SCYB4
• NCBI Protein Information: platelet factor 4
• UniProt Protein Name: Platelet factor 4
• Protein Family: Platelet factor
• UniProt Gene Name: PF4
• UniProt Synonym Gene Names: CXCL4; SCYB4; PF-4
• UniProt Entry Name: PLF4_HUMAN

NCBI Description

This gene encodes a member of the CXC chemokine family. This chemokine is released from the alpha granules of activated platelets in the form of a homotetramer which has high affinity for heparin and is involved in platelet aggregation. This protein is chemotactic for numerous other cell type and also functions as an inhibitor of hematopoiesis, angiogenesis and T-cell function. The protein also exhibits antimicrobial activity against Plasmodium falciparum. [provided by RefSeq, Oct 2014]

Uniprot Description

PF4: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Apoptosis; Chemokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q12-q21

Cellular Component: extracellular region; extracellular space

Molecular Function: chemokine activity; CXCR3 chemokine receptor binding; heparin binding; protein binding

Biological Process: cytokine and chemokine mediated signaling pathway; G-protein coupled receptor protein signaling pathway; immune response; inflammatory response; leukocyte chemotaxis; negative regulation of angiogenesis; negative regulation of cytolysis; negative regulation of megakaryocyte differentiation; negative regulation of MHC class II biosynthetic process; platelet activation; platelet degranulation; positive regulation of cAMP metabolic process; positive regulation of leukocyte chemotaxis; positive regulation of macrophage differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tumor necrosis factor production; regulation of cell proliferation; response to lipopolysaccharide