CXCL4/PF4 Recombinant Protein | PF4 recombinant protein

CXCL4/PF4 Protein, Human, Recombinant

Cat. Num. AAA8120537

• Gene Names: PF4; PF-4; CXCL4; SCYB4
• Purity: >80% as determined by SDS-PAGE
• Synonyms: CXCL4/PF4; CXCL4/PF4 Protein; Human; Recombinant; Human CXCL4/PF4 Protein; CXCL4 Protein; PF-4 Protein; SCYB4 Protein; platelet factor 4; PF4 recombinant protein

• Host: E Coli
• Purity/Purification: >80% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Glu32-Ser101

• Species: Human
• Predicted N Terminal: Met
• Tag: Native
• Protein Construction: A DNA sequence encoding the human CXCL4 (NP_002610.1)(Glu 32-Ser 101) was expressed.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PF4 recombinant protein

Background: Platelet factor 4 (PF4), also known as chemokine (C-X-C motif) ligand 4 (CXCL4), is a small cytokine belonging to the CXC chemokine family. CXCL4/PF4 is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, CXCL4/PF4 probably has a role in inflammation and wound repair. This protein is released during platelet aggregation. CXCL4/PF4 neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. CXCL4 is chemotactic for neutrophils and monocytes. It inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. CXCL4/PF4 is up-regulated in human liver fibrosis and that it plays a nonredundant, functional role in experimental liver fibrosis by mediating stellate cell proliferation, migration, and intrahepatic immune cell recruitment.

References

Zaldivar MM, et al. (2010) CXC chemokine ligand 4 (Cxcl4) is a platelet-derived mediator of experimental liver fibrosis. Hepatology. 51(4): 1345-53.Lasagni L, et al. (2007) PF-4/CXCL4 and CXCL4L1 exhibit distinct subcellular localization and a differentially regulated mechanism of secretion. Blood. 109(10): 4127-34.Struyf S, et al. (2004) Platelets release CXCL4L1, a nonallelic variant of the chemokine platelet factor-4/CXCL4 and potent inhibitor of angiogenesis. Circ Res. 95(9): 855-7.

NCBI and Uniprot Product Information

• NCBI GI #: 4505733
• NCBI GeneID: 5196
• NCBI Accession #: NP_002610.1
• NCBI GenBank Nucleotide #: NM_002619.3
• UniProt Accession #: P02776; Q53X61; Q9UC64; Q9UC65
• Molecular Weight: 10,845 Da
• NCBI Official Full Name: platelet factor 4
• NCBI Official Synonym Full Names: platelet factor 4
• NCBI Official Symbol: PF4
• NCBI Official Synonym Symbols: PF-4; CXCL4; SCYB4
• NCBI Protein Information: platelet factor 4; iroplact; oncostatin-A; C-X-C motif chemokine 4; chemokine (C-X-C motif) ligand 4
• UniProt Protein Name: Platelet factor 4
• Protein Family: Platelet factor
• UniProt Gene Name: PF4
• UniProt Synonym Gene Names: CXCL4; SCYB4; PF-4
• UniProt Entry Name: PLF4_HUMAN

NCBI Description

This gene encodes a member of the CXC chemokine family. This chemokine is released from the alpha granules of activated platelets in the form of a homotetramer which has high affinity for heparin and is involved in platelet aggregation. This protein is chemotactic for numerous other cell type and also functions as an inhibitor of hematopoiesis, angiogenesis and T-cell function. [provided by RefSeq, Mar 2012]

Uniprot Description

PF4: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Apoptosis; Chemokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q12-q21

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; CXCR3 chemokine receptor binding; chemokine activity

Biological Process: negative regulation of cytolysis; platelet activation; cytokine and chemokine mediated signaling pathway; leukocyte chemotaxis; response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; positive regulation of cAMP metabolic process; positive regulation of tumor necrosis factor production; negative regulation of megakaryocyte differentiation; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; platelet degranulation; negative regulation of MHC class II biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; immune response; inflammatory response; positive regulation of macrophage differentiation; blood coagulation