Goat Fibroblast surface protein ELISA Kit | FSP elisa kit

Goat Fibroblast surface protein ELISA Kit

Cat. Num. AAA746417

• Reactivity: Goat
• Synonyms: Fibroblast surface protein; Goat Fibroblast surface protein ELISA Kit; FSP elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This FSP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat FSP. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This FSP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat FSP. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FSP elisa kit

Principle of the Assay: FSP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FSP antibody and an FSP-HRP conjugate. The assay sample and buffer are incubated together with FSP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FSP concentration since FSP from samples and FSP-HRP conjugate compete for the anti-FSP antibody binding site. Since the number of sites is limited, as more sites are occupied by FSP from the sample, fewer sites are left to bind FSP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FSP concentration in each sample is interpolated from this standard curve.

Product Categories/Family for FSP elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 685015
• Molecular Weight: 46,233 Da
• NCBI Official Full Name: FSP
• UniProt Protein Name: Stress protein DDR48
• UniProt Gene Name: DDR48
• UniProt Synonym Gene Names: FSP; YMR173W; YM8010.03; DDRP 48
• UniProt Entry Name: DDR48_YEAST

Uniprot Description

DNA damage-responsive protein that may be required for maintaining the rate of spontaneous mutagenesis. Shows low ATP and GTP hydrolysis activity. Dispensable for acquisition of thermotolerance and does not play a significant role in recovery or protection of cells from acute heat shock.