SDS-Page
AKR7A3 active protein
AKR7A3, 1-331aa, Human, His tag, E. coli (Bioactivity Validated)
Gene Names
AKR7A3; AFAR2
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
AKR7A3; 1-331aa; Human; His tag; E. coli (Bioactivity Validated); Aldo-keto reductase family 7 member A 3; Aflatoxin B1 aldehyde reductase member 3; AFB1 aldehyde reductase 2 (AFAR2); Aldo-keto reductase family 7; member A 3 AFAR 2; AFAR2; AFB1 AR 2; Aflatoxin aldehyde reductase; Aflatoxin B1 aldehyde reductase 2; Aldo keto reductase family 7 member A3; OTTHuMP00000002623.; AKR7A3 active protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 100mM NaCl, 10% glycerol
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELEM SRQLSRARPA TVLGAMEMGR RMDAPTSAAV TRAFLERGHT EIDTAFVYSE GQSETILGGL GLRLGGSDCR VKIDTKAIPL FGNSLKPDSL RFQLETSLKR LQCPRVDLFY LHMPDHSTPV EETLRACHQL HQEGKFVELG LSNYAAWEVA EICTLCKSNG WILPTVYQGM YNAITRQVET ELFPCLRHFG LRFYAFNPLA GGLLTGKYKY EDKDGKQPVG RFFGNTWAEM YRNRYWKEHH FEGIALVEKA LQAAYGASAP SMTSATLRWM YHHSQLQGAH GDAVILGMSS LEQLEQNLAA AEEGPLEPAV VDAFNQAWHL VAHECPNYFR
Sequence Length
331
Applicable Applications for AKR7A3 active protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Biological Activity
Specific activity is > 700 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0 pmole of 1,2-Naphthoquinone presence of NADPH per minute at pH 7.0 at 25C.
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for AKR7A3 active protein
Aldo-keto reductase family 7, member A 3, also known as AKR7A3, is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member includes a number of related monomeric NADPH-dependent oxidoreductases, such as aldehyde reductase, aldose reductase, prostaglandin F synthase, xylose reductase, rho crystallin, and many others. AKR7A3 is involved in the detoxification of aldehydes and ketones. The activity of AKR7A3 may detoxify the aflatoxin B1 (AFB1) dialdehyde, which reacts with proteins, and thereby inhibits AFB 1 induced toxicity. Recombinant human AKR7A3, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for AKR7A3 active protein
References
Kniqht LP., et al. (1999) Carcinogenesis. 20 (7):1215-23. ; Bodreddiqari S., et al. (2008) Chem Res Toxicol. 21 (5):1134-42.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
41.6 kDa (370aa), confirmed by MALDI-TOF.
NCBI Official Full Name
Aldo-keto reductase family 7, member A3 (aflatoxin aldehyde reductase)
NCBI Official Synonym Full Names
aldo-keto reductase family 7 member A3
NCBI Official Symbol
AKR7A3
NCBI Official Synonym Symbols
AFAR2
NCBI Protein Information
aflatoxin B1 aldehyde reductase member 3
UniProt Protein Name
Aflatoxin B1 aldehyde reductase member 3
Protein Family
UniProt Gene Name
AKR7A3
UniProt Synonym Gene Names
AFAR2; AFB1-AR 2
NCBI Description
Aldo-keto reductases, such as AKR7A3, are involved in the detoxification of aldehydes and ketones.[supplied by OMIM, Apr 2004]
Uniprot Description
Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen.
Research Articles on AKR7A3
Similar Products
Product Notes
The AKR7A3 akr7a3 (Catalog #AAA203611) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's AKR7A3 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the AKR7A3 akr7a3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELEM SRQLSRARPA TVLGAMEMGR RMDAPTSAAV TRAFLERGHT EIDTAFVYSE GQSETILGGL GLRLGGSDCR VKIDTKAIPL FGNSLKPDSL RFQLETSLKR LQCPRVDLFY LHMPDHSTPV EETLRACHQL HQEGKFVELG LSNYAAWEVA EICTLCKSNG WILPTVYQGM YNAITRQVET ELFPCLRHFG LRFYAFNPLA GGLLTGKYKY EDKDGKQPVG RFFGNTWAEM YRNRYWKEHH FEGIALVEKA LQAAYGASAP SMTSATLRWM YHHSQLQGAH GDAVILGMSS LEQLEQNLAA AEEGPLEPAV VDAFNQAWHL VAHECPNYFR. It is sometimes possible for the material contained within the vial of "AKR7A3, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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