AKR7A3 active protein

AKR7A3, 1-331aa, Human, E Coli (Bioactivity Validated)

Cat. Num. AAA206300

• Gene Names: AKR7A3; AFAR2
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: AKR7A3; 1-331aa; Human; E Coli (Bioactivity Validated); Aldo-keto reductase family 7; member A 3; Aflatoxin B1 aldehyde reductase member 3; AFB1 aldehyde reductase 2 (AFAR2); AKR7A3 active protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.5) containing 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MSRQLSRARP ATVLGAMEMG RRMDAPTSAA VTRAFLERGH TEIDTAFVYS EGQSETILGG LGLRLGGSDC RVKIDTKAIP LFGNSLKPDS LRFQLETSLK RLQCPRVDLF YLHMPDHSTP VEETLRACHQ LHQEGKFVEL GLSNYAAWEV AEICTLCKSN GWILPTVYQG MYNAITRQVE TELFPCLRHF GLRFYAFNPL AGGLLTGKYK YEDKDGKQPV GRFFGNTWAE MYRNRYWKEH HFEGIALVEK ALQAAYGASA PSMTSATLRW MYHHSQLQGA HGDAVILGMS SLEQLEQNLA AAEEGPLEPA VVDAFNQAWH LVAHECPNYF R
• Sequence Length: 331

• Applicable Applications for AKR7A3 active protein: SDS-PAGE
• Antigen Species: Human
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Biological Activity: Specific activity is > 800 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0 pmole of 1,2-Naphthoquinone presence of NADPH per minute at pH 7.0 at 25C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for AKR7A3 active protein

Aldo-keto reductase family 7, member A 3, also known as AKR7A3, is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member includes a number of related monomeric NADPH-dependent oxidoreductases, such as aldehyde reductase, aldose reductase, prostaglandin F synthase, xylose reductase, rho crystallin, and many others. AKR7A3 is involved in the detoxification of aldehydes and ketones. The activity of AKR7A3 may detoxify the aflatoxin B1 (AFB1) dialdehyde, which reacts with proteins, and thereby inhibits AFB 1 induced toxicity. Recombinant human AKR7A3 was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for AKR7A3 active protein

Metabolism

References

Ahmed MM., et al. (2011) Hepatology. 54 (4):1322-32. ; Bodreddiqari S., et al. (2008) Chem Res Toxicol. 21 (5):1134-42.

NCBI and Uniprot Product Information

• NCBI GI #: 19343681
• NCBI GeneID: 22977
• Molecular Weight: 37.7 kDa (331aa)
• NCBI Official Full Name: Aldo-keto reductase family 7, member A3 (aflatoxin aldehyde reductase)
• NCBI Official Synonym Full Names: aldo-keto reductase family 7 member A3
• NCBI Official Symbol: AKR7A3
• NCBI Official Synonym Symbols: AFAR2
• NCBI Protein Information: aflatoxin B1 aldehyde reductase member 3
• UniProt Protein Name: Aflatoxin B1 aldehyde reductase member 3
• Protein Family: Aflatoxin B1 aldehyde reductase
• UniProt Gene Name: AKR7A3
• UniProt Synonym Gene Names: AFAR2; AFB1-AR 2

NCBI Description

Aldo-keto reductases, such as AKR7A3, are involved in the detoxification of aldehydes and ketones.[supplied by OMIM, Apr 2004]

Uniprot Description

Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen.