MMP-2 Recombinant Protein | MMP2 recombinant protein

MMP-2 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8123237

• Gene Names: Mmp2; GelA; Clg4a; MMP-2
• Purity: >95% as determined by SDS-PAGE
• Synonyms: MMP-2; MMP-2 Protein; Mouse; Recombinant (His Tag); Mouse MMP-2/MMP2 Protein (His Tag); Clg4a Protein; GelA Protein; matrix metallopeptidase 2; MMP2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met409-Cys662

• Species: Mouse
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mouse Mmp2 (NP_032636.1)(Met409-Cys662) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for MMP2 recombinant protein

Background: Matrix Metalloproteinase-2 (MMP-2) is an enzyme that degrades components of the extracellular matrix and thus plays a pivotal role in cell migration during physiological and pathological processes. MMP-2 expression is dependent on extracellular matrix metalloproteinase inducer (EMMPRIN), Her2/neu, growth factors, cytokines, and hormones. Pro-MMP-2 activation needs MT1-MMP and TIMP-2 contribution. MMP-2 is changed in distribution and increased in amount in the ventral cochlear nucleus after unilateral cochlear ablation. A low level of MMP-2 is linked to a favorable prognosis in patients with a hormone receptor-negative tumor, usually associated with high risk. As a zymogen requiring proteolytic activation for catalytic activity, MMP-2 has been implicated broadly in the invasion and metastasis of many cancer model systems, including human breast cancer (HBC). Blocking MMP-2 secretion and activation during breast carcinoma development may decrease metastasis. The detection of active MMP-2 alone or the rate of pro-MMP-2 and active MMP-2 is considered a very sensitive indicator of cancer metastasis. Modulation of MMP-2 expression and activation through specific inhibitors and activators may thus provide a new mechanism for breast cancer treatment.

Product Categories/Family for MMP2 recombinant protein

Cancer Drug Targets

References

Thompson EW, et al. (1994) Collagen induced MMP-2 activation in human breast cancer. Breast Cancer Res Treat. 31(2-3): 357-70.Jezierska A, et al. (2009) Matrix metalloproteinase-2 involvement in breast cancer progression: a mini-review. Med Sci Monit. 15(2): RA32-40.Fredrich M, et al. (2010) MMP-2 is involved in synaptic remodeling after cochlear lesion. Neuroreport. 21(5): 324-7.

NCBI and Uniprot Product Information

• NCBI GI #: 6678902
• NCBI GeneID: 17390
• NCBI Accession #: NP_032636.1
• NCBI GenBank Nucleotide #: NM_008610.3
• UniProt Accession #: P33434
• Molecular Weight: 65,883 Da
• NCBI Official Full Name: 72 kDa type IV collagenase preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 2
• NCBI Official Symbol: Mmp2
• NCBI Official Synonym Symbols: GelA; Clg4a; MMP-2
• NCBI Protein Information: 72 kDa type IV collagenase
• UniProt Protein Name: 72 kDa type IV collagenase
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: Mmp2
• UniProt Synonym Gene Names: MMP-2
• UniProt Entry Name: MMP2_MOUSE

NCBI Description

This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme that hydrolyzes collagens, gelatins, laminin, fibronectin and elastin. Mice lacking the encoded protein exhibit suppressed angiogenesis and attenuated features of human multicentric osteolysis with arthritis including abnormal skeletal and craniofacial development. [provided by RefSeq, Feb 2016]

Uniprot Description

MMP2: Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Interacts (via the C-terminal hemopexin-like domains- containing region) with the integrin alpha-V/beta-3; the interaction promotes vascular invasion in angiogenic vessels and melamoma cells. Interacts (via the C-terminal PEX domain) with TIMP2 (via the C-terminal); the interaction inhibits the degradation activity. Interacts with GSK3B. Aspirin appears to inhibit expression. Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate. Inhibited by histatin-3 1/24 (histatin-5). Belongs to the peptidase M10A family.

Protein type: Protease; Secreted; Apoptosis; Cell development/differentiation; EC 3.4.24.24; Secreted, signal peptide; Motility/polarity/chemotaxis

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; sarcomere; membrane; mitochondrion; cytoplasm; plasma membrane; extracellular region; nucleus

Molecular Function: peptidase activity; zinc ion binding; hydrolase activity; metallopeptidase activity; metal ion binding; metalloendopeptidase activity; fibronectin binding

Biological Process: response to drug; cell migration; intramembranous ossification; positive regulation of apoptosis; tissue remodeling; negative regulation of cell adhesion; proteolysis; axon regeneration in the peripheral nervous system; blood vessel maturation; negative regulation of vasoconstriction; collagen catabolic process; response to hydrogen peroxide; ovarian follicle development; response to estrogen stimulus; skeletal morphogenesis; response to mechanical stimulus; positive regulation of innate immune response; response to hypoxia; response to oxidative stress; angiogenesis; embryo implantation; positive regulation of cell migration