AKR7A2 active protein

AKR7A2, 1-359aa, Human, His tag, E. coli (Bioactivity Validated)

Cat. Num. AAA203414

• Gene Names: AKR7A2; AFAR; AKR7; AFAR1; AFB1-AR1
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: AKR7A2; 1-359aa; Human; His tag; E. coli (Bioactivity Validated); aldo-keto reductase family 7 member A2; Aflatoxin B1 aldehyde reductase member 2; AFAR; AFAR1; AFB1-AR1; AKR7; aldo-keto reductase family 7; member A2 AFB1 AR1; AFB1AR1; aflatoxin aldehyde reductase; aflatoxin beta1 aldehyde reductase; Aiar; aldo keto reductase family 7; member A2; aldoketoreductase 7.; AKR7A2 active protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 20% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELEM LSAASRVVSR AAVHCALRSP PPEARALAMS RPPPPRVASV LGTMEMGRRM DAPASAAAVR AFLERGHTEL DTAFMYSDGQ SETILGGLGL GLGGGDCRVK IATKANPWDG KSLKPDSVRS QLETSLKRLQ CPQVDLFYLH APDHGTPVEE TLHACQRLHQ EGKFVELGLS NYASWEVAEI CTLCKSNGWI LPTVYQGMYN ATTRQVETEL FPCLRHFGLR FYAYNPLAGG LLTGKYKYED KDGKQPVGRF FGNSWAETYR NRFWKEHHFE AIALVEKALQ AAYGASAPSV TSAALRWMYH HSQLQGAHGD AVILGMSSLE QLEQNLAATE EGPLEPAVVD AFNQAWHLVA HECPNYFR
• Sequence Length: 359

• Applicable Applications for AKR7A2 active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Biological Activity: Specific activity is > 1000 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0 pmole of 1,2-Naphthoquinone presence of NADPH per minute at pH 7.0 at 25C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for AKR7A2 active protein

Aldo-keto reductases, such as AKR7A2, are involved in the detoxification of aldehydes and ketones. This protein can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the nonbinding AFB1 dialcohol. It may be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen. Also, it has been proposed previously to catalyze the NADPH-dependent reduction of succinic semialdehyde (SSA) to gamma-Hydroxybutyrate in human brain. Recombinant AKR7A2 protein was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for AKR7A2 active protein

Redox Proteins

References

Bodreddigari S., et al. (2008) Chem Res Toxicol. 21 (5):1134-42.; Malaspina P., et al. (2009) Hum Genomics. 3 (2):106-20.

NCBI and Uniprot Product Information

• NCBI GI #: 41327764
• NCBI GeneID: 8574
• NCBI Accession #: NP_003680
• NCBI GenBank Nucleotide #: NM_003689.3
• UniProt Accession #: O43488; O75749; Q5TG63
• Molecular Weight: 44.0kDa (398aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: aflatoxin B1 aldehyde reductase member 2 isoform 1
• NCBI Official Synonym Full Names: aldo-keto reductase family 7 member A2
• NCBI Official Symbol: AKR7A2
• NCBI Official Synonym Symbols: AFAR; AKR7; AFAR1; AFB1-AR1
• NCBI Protein Information: aflatoxin B1 aldehyde reductase member 2
• UniProt Protein Name: Aflatoxin B1 aldehyde reductase member 2
• Protein Family: Aflatoxin B1 aldehyde reductase
• UniProt Gene Name: AKR7A2
• UniProt Synonym Gene Names: AFAR; AFAR1; AKR7; AFB1-AR 1; SSA reductase

NCBI Description

The protein encoded by this gene belongs to the aldo/keto reductase (AKR) superfamily and AKR7 family, which are involved in the detoxification of aldehydes and ketones. The AKR7 family consists of 3 genes that are present in a cluster on the p arm of chromosome 1. This protein, thought to be localized in the golgi, catalyzes the NADPH-dependent reduction of succinic semialdehyde to the endogenous neuromodulator, gamma-hydroxybutyrate. It may also function as a detoxication enzyme in the reduction of aflatoxin B1 and 2-carboxybenzaldehyde. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Uniprot Description

Catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate. May have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB). Has broad substrate specificity. Has NADPH-dependent aldehyde reductase activity towards 2-carboxybenzaldehyde, 2-nitrobenzaldehyde and pyridine-2-aldehyde (in vitro). Can reduce 1,2-naphthoquinone and 9,10-phenanthrenequinone (in vitro). Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen.