AKR1C1 active protein

Human Recombinant AKR1C1

Cat. Num. AAA844689

• Gene Names: AKR1C1; C9; DD1; DDH; DDH1; H-37; HBAB; MBAB; HAKRC; DD1/DD2; 2-ALPHA-HSD; 20-ALPHA-HSD
• Applications: SDS-Page
• Purity: >=90%
• Synonyms: AKR1C1; Human Recombinant AKR1C1; Aldo-keto reductase family 1; member C 1; 20-alpha-HSD; DD1/DD2; HBAB; DDH; DDH1; AKR1C1 active protein

• Host: E Coli
• Purity/Purification: >=90%
• Form/Format: 0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT and 20% glycerol.; Appearance: Liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEATKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA VEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN RNVRYLTLDI FAGPPNYPFS DEY
• Sequence Length: 323

• Applicable Applications for AKR1C1 active protein: SDS-PAGE
• Biological Activity: Specific activity is approximately 0.15 - 0.2 units/mg.
• Unit Definition: Specific activity is approximately 0.15 - 0.2 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25 degree C. Specific activity was expressed as units/mg protein.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -80 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for AKR1C1 active protein

Background: Aldo-keto reductase family 1 member C1 in humans is encoded by the AKR1C1 gene. AKR1C1 transfers progesterone to its inactive state or in other words catalyzes the reaction of 20-alpha-hydroxy progesterone (20-alpha-OHP) in the liver and intestine. AKR1C1 transfers bile and monitors the intrahepatic bile acid concentration though it has a low bile-binding ability. AKR1C1 participates in myelin formation. AKR1C1 is part of the aldo/keto reductase superfamily, which has over 40 known enzymes which catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors thus display overlapping but distinct substrate specificity.

Product Categories/Family for AKR1C1 active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)  

NCBI and Uniprot Product Information

• NCBI GI #: 5453543
• NCBI GeneID: 1645
• NCBI Accession #: NP_001344.2
• NCBI GenBank Nucleotide #: NM_001353.5
• UniProt Accession #: Q04828; P52896; Q5SR15; Q7M4N2; Q9UCX2
• Molecular Weight: 38.9 kDa
• NCBI Official Full Name: aldo-keto reductase family 1 member C1
• NCBI Official Synonym Full Names: aldo-keto reductase family 1, member C1
• NCBI Official Symbol: AKR1C1
• NCBI Official Synonym Symbols: C9; DD1; DDH; DDH1; H-37; HBAB; MBAB; HAKRC; DD1/DD2; 2-ALPHA-HSD; 20-ALPHA-HSD
• NCBI Protein Information: aldo-keto reductase family 1 member C1
• UniProt Protein Name: Aldo-keto reductase family 1 member C1
• Protein Family: Aldo-keto reductase family
• UniProt Gene Name: AKR1C1
• UniProt Synonym Gene Names: DDH; DDH1; 20-alpha-HSD; DD1/DD2; HBAB
• UniProt Entry Name: AK1C1_HUMAN

NCBI Description

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq, Jul 2008]

Uniprot Description

AKR1C1: Converts progesterone to its inactive form, 20-alpha- dihydroxyprogesterone (20-alpha-OHP). In the liver and intestine, may have a role in the transport of bile. May have a role in monitoring the intrahepatic bile acid concentration. Has a low bile-binding ability. May play a role in myelin formation. Belongs to the aldo/keto reductase family.

Protein type: EC 1.1.1.112; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.1.1.149; EC 1.3.1.20; Oxidoreductase

Chromosomal Location of Human Ortholog: 10p15-p14

Cellular Component: cytosol

Molecular Function: oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; ketosteroid monooxygenase activity; 3-alpha-hydroxysteroid dehydrogenase (B-specific) activity; carboxylic acid binding; bile acid binding; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity; aldo-keto reductase activity; 20-alpha-hydroxysteroid dehydrogenase activity; indanol dehydrogenase activity; aldehyde reductase activity; phenanthrene 9,10-monooxygenase activity

Biological Process: bile acid and bile salt transport; phototransduction, visible light; cholesterol homeostasis; response to organophosphorus; retinal metabolic process; epithelial cell differentiation; bile acid metabolic process; xenobiotic metabolic process; digestion; cholesterol absorption; progesterone metabolic process; retinoid metabolic process; protein homooligomerization