Western Blot (WB)
(Western blot analysis of AKR1C1/C2 expression in rat liver extract (lane 1) and HELA whole cell lysates (lane 2). AKR1C1/C2 at 37KD was detected using rabbit anti- AKR1C1/C2 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Rabbit anti-Human, Rat AKR1C1/C2 Polyclonal Antibody | anti-AKR1C1/C2 antibody
Anti-AKR1C1/C2 Antibody
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Other applications have not been tested.
Western Blot (WB)
(Western blot analysis of AKR1C1/C2 expression in rat liver extract (lane 1) and HELA whole cell lysates (lane 2). AKR1C1/C2 at 37KD was detected using rabbit anti- AKR1C1/C2 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Western Blot (WB)
(Western blot analysis of AKR1C1/C2 expression in rat liver extract (lane 1) and HELA whole cell lysates (lane 2). AKR1C1/C2 at 37KD was detected using rabbit anti- AKR1C1/C2 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Background: This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. And this gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene.
2. O'connor T, Ireland LS, Harrison DJ, Hayes JD (Oct 1999). "Major differences exist in the function and tissue-specific expression of human aflatoxin B1 aldehyde reductase and the principal human aldo-keto reductase AKR1 family members". The Biochemical Journal 343 (2): 487-504.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq, Jul 2008]
Uniprot Description
AKR1C1: Converts progesterone to its inactive form, 20-alpha- dihydroxyprogesterone (20-alpha-OHP). In the liver and intestine, may have a role in the transport of bile. May have a role in monitoring the intrahepatic bile acid concentration. Has a low bile-binding ability. May play a role in myelin formation. Belongs to the aldo/keto reductase family.
Protein type: EC 1.1.1.112; EC 1.1.1.149; EC 1.3.1.20; Oxidoreductase; Xenobiotic Metabolism - metabolism by cytochrome P450
Chromosomal Location of Human Ortholog: 10p15.1
Cellular Component: cytosol
Molecular Function: 3-alpha-hydroxysteroid dehydrogenase (B-specific) activity; aldehyde reductase activity; aldo-keto reductase activity; bile acid binding; carboxylic acid binding; ketosteroid monooxygenase activity; oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; phenanthrene 9,10-monooxygenase activity; protein binding; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity
Biological Process: bile acid and bile salt transport; bile acid metabolic process; cholesterol absorption; cholesterol homeostasis; digestion; epithelial cell differentiation; progesterone metabolic process; protein homooligomerization; response to organophosphorus; retinal metabolic process; retinoid metabolic process
Research Articles on AKR1C1/C2
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Product Notes
The AKR1C1/C2 akr1c1 (Catalog #AAA178792) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-AKR1C1/C2 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's AKR1C1/C2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the AKR1C1/C2 akr1c1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AKR1C1/C2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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