Testing Data
(Active alpha synuclein aggregate (SPR-324) seeds the formation of new alpha Synuclein aggregates from the pool of active alpha Synuclein monomers (SPR-323). Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate (SPR-324) is combined with 100 uM of active alpha Synuclein monomer (SPR-323), as compared to active alpha Synuclein aggregate (SPR-324) and active alpha Synuclein monomer (SPR-323) alone. Thioflavin T ex = 450 nm, em = 485 nm.)
Alpha Synuclein Active Protein | Snca active protein
Alpha Synuclein Protein
Gene Names
Snca; NACP; alphaSYN; alpha-Syn
Applications
Western Blot, SDS-Page
Purity
>95%Ion-exchange Purified
Synonyms
Alpha Synuclein; Alpha Synuclein Protein; Active Mouse Recombinant Alpha Synuclein Protein Monomer; Alpha synuclein pre-formed fibrils; Alpha synuclein aggregates; Alpha synuclein protein aggregates; Active Alpha synuclein aggregates; Alpha-synuclein protein; Non-A beta component of AD amyloid protein; Non-A4 component of amyloid precursor protein; NACP protein; SNCA protein; PARK1 protein; Alpha synuclein monomers; SYN protein; Parkison disease familial 1 Protein; Snca active protein
Host
E Coli
Specificity
~14.46 kDa
Purity/Purification
>95%
Ion-exchange Purified
Ion-exchange Purified
Sequence
MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVTTVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGNIAA ATGFVKKDQM GKGEEGYPQE GILEDMPVDP GSEAYEMPSE EGYQDYEPEA
Sequence Length
Full Length
Applicable Applications for Snca active protein
Western Blot (WB), SDS-PAGE, In vivo assay, In vitro assay
Species
Mouse
Target
Alpha Synuclein
Cellular Localization
Cytoplasm; Membrane; Nucleus
Storage Buffer
PBS pH 7.4
Biological Activity
100 uM alpha synuclein protein monomer seeded with 10 nM alpha synuclein protein aggregate in 25 uM Thioflavin T (PBS pH 7.4, 100 ul reaction volume) generated an increased fluorescence intensity after incubation at 37 degree C with shaking at 600 rpm for 24 hours. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.
Tag Information
No tag
Preparation and Storage
Store at -80 degree C.
Testing Data
(Active alpha synuclein aggregate (SPR-324) seeds the formation of new alpha Synuclein aggregates from the pool of active alpha Synuclein monomers (SPR-323). Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate (SPR-324) is combined with 100 uM of active alpha Synuclein monomer (SPR-323), as compared to active alpha Synuclein aggregate (SPR-324) and active alpha Synuclein monomer (SPR-323) alone. Thioflavin T ex = 450 nm, em = 485 nm.)
Testing Data
(Active alpha synuclein aggregate (SPR-324) seeds the formation of new alpha Synuclein aggregates from the pool of active alpha Synuclein monomers (SPR-323). Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate (SPR-324) is combined with 100 uM of active alpha Synuclein monomer (SPR-323), as compared to active alpha Synuclein aggregate (SPR-324) and active alpha Synuclein monomer (SPR-323) alone. Thioflavin T ex = 450 nm, em = 485 nm.)
SDS-Page
(SDS-PAGE of ~14 kDa Active Mouse Recombinant Alpha Synuclein Protein Monomer (SPR-323). Lane 1: Molecular Weight Ladder (MW). Lane 2: Active Alpha Synuclein Protein Monomer (2 ug) (SPR-323).)
SDS-Page
(SDS-PAGE of ~14 kDa Active Mouse Recombinant Alpha Synuclein Protein Monomer (SPR-323). Lane 1: Molecular Weight Ladder (MW). Lane 2: Active Alpha Synuclein Protein Monomer (2 ug) (SPR-323).)
Related Product Information for Snca active protein
Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions; inactivation may lead to impaired spatial learning and working memory (4).
SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6).
SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6).
Product Categories/Family for Snca active protein
References
1. "Genetics Home Reference: SNCA". US National Library of Medicine. (2013). 2. Zhang L., et al. (2008) Brain Res. 1244: 40-52. 3. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117. 4. Kokhan V.S., Afanasyeva M.A., Van'kin G. (2012) Behav. Brain. Res. 231(1): 226-230. 5. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840. 6. Mezey E., et al. (1998) Nat Med. 4(7): 755-757.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
12,345 Da
NCBI Official Full Name
alpha-synuclein
NCBI Official Synonym Full Names
synuclein, alpha
NCBI Official Symbol
Snca
NCBI Official Synonym Symbols
NACP; alphaSYN; alpha-Syn
NCBI Protein Information
alpha-synuclein
UniProt Protein Name
Alpha-synuclein
Protein Family
UniProt Gene Name
Snca
UniProt Synonym Gene Names
Syn; NACP
Uniprot Description
May be involved in the regulation of dopamine release and transport.
Research Articles on Snca
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Product Notes
The Snca snca (Catalog #AAA8001847) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is No tag. AAA Biotech's Alpha Synuclein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), SDS-PAGE, In vivo assay, In vitro assay. Researchers should empirically determine the suitability of the Snca snca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVTTVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGNIAA ATGFVKKDQM GKGEEGYPQE GILEDMPVDP GSEAYEMPSE EGYQDYEPEA. It is sometimes possible for the material contained within the vial of "Alpha Synuclein, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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