Typical Testing Data/Standard Curve (for reference only)
Human Histone H2B (H2B) ELISA Kit | H2B elisa kit
Human Histone H2B (H2B) ELISA Kit
Gene Names
HTB2; H2B; histone B2; HISTONE H2B; HTB2; MRN17.11; MRN17_11
Reactivity
Human
Synonyms
Histone H2B (H2B); Human Histone H2B (H2B) ELISA Kit; H2B elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity for detection of H2B. 100% cross-reactivity of H2B was observed among human, mouse and rat.
Sequence Length
145
Samples
Tissue homogenates, cell lysates and other biological fluids. due to the 100% homology of the sequence among different species, the kit can be used to detect human, mouse and rat samples
Assay Type
Quantitative Sandwich
Detection Range
3.12-200ng/mL
Sensitivity
< 1.13ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level H2B were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level H2B were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for H2B elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of H2B in tissue homogenates, cell lysates and other biological fluids. Due to the 100% homology of the sequence among different species, the kit can be used to detect human, mouse and rat samples.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to H2B. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to H2B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain H2B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of H2B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to H2B. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to H2B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain H2B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of H2B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for H2B elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,732 Da
NCBI Official Full Name
histone H2B
NCBI Official Symbol
HTB2
NCBI Official Synonym Symbols
H2B; histone B2; HISTONE H2B; HTB2; MRN17.11; MRN17_11
NCBI Protein Information
histone H2B
UniProt Protein Name
Histone H2B.10
UniProt Gene Name
At5g22880
UniProt Entry Name
H2B10_ARATH
Uniprot Description
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Research Articles on H2B
Similar Products
Product Notes
The Human H2B at5g22880 (Catalog #AAA2024750) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2024750 ELISA Kit recognizes Human H2B. It is sometimes possible for the material contained within the vial of "Histone H2B (H2B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
