Rabbit Alpha Synuclein Polyclonal Antibody | anti-SNCA antibody

Alpha Synuclein Antibody: PerCP

Cat. Num. AAA8002063

• Gene Names: SNCA; PD1; NACP; PARK1; PARK4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: Alpha Synuclein; Polyclonal Antibody; Alpha Synuclein Antibody: PerCP; Rabbit Anti-Human Alpha Synuclein Polyclonal; Alpha Synuclein Antibody; Non-A4 component of amyloid precursor Antibody; MGC105443 Antibody; OTTHUMP00000218552 Antibody; MGC110988 Antibody; OTTHUMP00000218553 Antibody; Synuclein alpha 140 Antibody; alphaSYN Antibody; Parkinson disease familial 1 Antibody; OTTHUMP00000218554 Antibody; Parkinson disease (autosomal dominant; Lewy body) 4 Antibody; Synuclein; alpha (non A4 component of amyloid precursor) Antibody; OTTHUMP00000218549 Antibody; Non A4 component of amyloid precursor Antibody; MGC127560 Antibody; SYN Antibody; SNCA Antibody; MGC64356 Antibody; Non A4 component of amyloid Antibody; PARK4 Antibody; Alpha synuclein Antibody; PARK 1 Antibody; Snca synuclein; Synuclein alpha Antibody; Non-A4 component of amyloid; precursor of Antibody; OTTHUMP00000218551 Antibody; PARK 4 Antibody; PARK1 Antibody; Alpha-synuclein; isoform NACP140 Antibody; Non-A-beta component of alzheimers disease amyloid; Non-A beta component of AD amyloid Antibody; NACP Antibody; SYUA_HUMAN Antibody; Alpha-synuclein Antibody; Non A beta component of AD amyloid Antibody; anti-SNCA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Detects ~14.46kda. Highly expressed in brain (presynaptic nerve terminals) but is also expressed in low concentrations in all tissues examined except in liver.
• Purity/Purification: Protein A Purified
• Form/Format: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 140

• Applicable Applications for anti-SNCA antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000
• Immunogen: Synthetic peptide from the full length of Human Alpha Synuclein
• Immunogen Species: Human
• Conjugate: PerCP
• Cellular Localization: Cytoplasm, Membrane, Nucleus, Cell Junction, Synapse, Secreted
• Certificate of Analysis: A 1:1000 dilution was sufficient for detection of Alpha Synuclein in 15 mug of mouse Brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
• Preparation and Storage: Store at-20 degree C.

Western Blot (WB)

(Western blot analysis of Mouse Brain showing detection of ~14.46kDa Alpha Synuclein protein using Rabbit Anti-Alpha Synuclein Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Alpha Synuclein Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~14.46kDa. Other Band(s): Higher molecular weight oligomers.)

Related Product Information for anti-SNCA antibody

Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions; inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6).

Product Categories/Family for anti-SNCA antibody

Neuroscience; Neurodegeneration; Alzheimer's Disease; Tangles & Tau; Neuroscience; Neurodegeneration; Parkinson's Disease; Synuclein

References

1. "Genetics Home Reference: SNCA". US National Library of Medicine. (2013). 2. Zhang L., et al. (2008) Brain Res. 1244: 40-52. 3. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117. 4. Kokhan V.S., Afanasyeva M.A., Van'kin G. (2012) Behav. Brain. Res. 231(1): 226-230. 5. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840. 6. Mezey E., et al. (1998) Nat Med. 4(7): 755-757.

NCBI and Uniprot Product Information

• NCBI GI #: 4507109
• NCBI GeneID: 6622
• NCBI Accession #: NP_000336.1
• NCBI GenBank Nucleotide #: NM_000345.3
• UniProt Accession #: P37840 ; P37840
• NCBI Official Full Name: alpha-synuclein isoform NACP140
• NCBI Official Synonym Full Names: synuclein alpha
• NCBI Official Symbol: SNCA
• NCBI Official Synonym Symbols: PD1; NACP; PARK1; PARK4
• NCBI Protein Information: alpha-synuclein
• Protein Family: Alpha-synuclein

NCBI Description

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016]