DNMT3A/DNMT3L recombinant protein

Recombinant DNMT3A/DNMT3L complex

Cat. Num. AAA388133

• Gene Names: DNMT3L; MGC1090
• Purity: The recombinant protein is >95% pure by SDS-PAGE.
• Synonyms: DNMT3A/DNMT3L; Recombinant DNMT3A/DNMT3L complex; DNMT3A/DNMT3L recombinant protein

• Host: Baculovirus
• Purity/Purification: The recombinant protein is >95% pure by SDS-PAGE.
• Form/Format: Recombinant DNMT3A/DNMT3L complex is supplied in 25mM Hepes pH7.5, 300mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2mM TCEP and 0.2mg/ml 3× DYKDDDDK peptide. Please refer to product insert upon arrival for lot-specific concentration.

• Protein Species: Human
• Tag: DYKDDDDK-Tag, His-Tag
• Notes: Recombinant DNMT3A/DNMT3L complex is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling. DNMT Activity Assay Conditions: Assay was performed using DNMT Activity/Inhibition Assay, for 1 hour at 37 degree C.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant DNMT3A / DNMT3L complex gel Recombinant DNMT3A / DNMT3L complex was run on an 8% SDS-PAGE gel and stained with Coomassie blue.)

Testing Data

(Recombinant DNMT3A / DNMT3L complex activity assay Recombinant DNMT3A / DNMT3L complex activity was measured using DNMT Activity / Inhibition Assay, for 1 hour at 37 degree C)

Related Product Information for DNMT3A/DNMT3L recombinant protein

Short Description: Recombinant DNMT3A/DNMT3L complex that includes human DNMT3A amino acids 214-912 (accession number NP_783328.1) with an N-terminal 6xHis-tag and DYKDDDDK-Tag and full-length DNMT3L (accession number NP_787063.1) with an N-terminal 6xHis-tag was expressed in Sf9 cells. The molecular weights of DNMT3A and DNMT3L are 83.8 kDa and 46.6 kDa, respectively. The recombinant protein is >=95% pure by SDS-PAGE. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Background: DNA methyltransferase proteins such as DNMT3A are involved in DNA methylation, in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. DNMT3A has been shown to be important in the regulation of specific patterns of DNA methylation. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and control of gene expression. DNA methylation is generally associated with transcriptional repression. Three families of DNMTs have been identified: DNMT1, DNMT2 and DNMT3. The DNMT3 family, containing two active methyltransferases, DNMT3A & DNMT3B, and one DNMT3-Like protein (DNMT3L), establishes the initial CpG methylation pattern de novo and shows the same propensity for methylating unmethylated duplex DNA as for hemi-methylated DNA. DNMT3A and DNMT3B are encoded by two distinct genes and are strongly expressed in embryonic stem cells. DNMT3A is ubiquitous in mammalian cells. DNMT3L is a nuclear protein with similarity to the DNMT family of proteins, but does not function as a DNA methyltransferase. It serves as an essential regulator of DNMT3A and DNMT3B, recruiting them to sites for deposition of de novo DNA methylation.

Product Categories/Family for DNMT3A/DNMT3L recombinant protein

Recombinant Proteins; DNA Methyltransferase (DNMT)

NCBI and Uniprot Product Information

• NCBI GI #: 28872780
• NCBI GeneID: 29947
• NCBI Accession #: NP_787063.1
• NCBI GenBank Nucleotide #: NM_175867.1
• UniProt Accession #: Q9UJW3; Q9BUJ4; E9PB42
• Molecular Weight: 43,583 Da
• NCBI Official Full Name: DNA (cytosine-5)-methyltransferase 3-like isoform 2
• NCBI Official Synonym Full Names: DNA (cytosine-5-)-methyltransferase 3-like
• NCBI Official Symbol: DNMT3L
• NCBI Official Synonym Symbols: MGC1090
• NCBI Protein Information: DNA (cytosine-5)-methyltransferase 3-like; DNA (cytosine-5)-methyltransferase 3-like; OTTHUMP00000109516; OTTHUMP00000109517; cytosine-5-methyltransferase 3-like protein; human cytosine-5-methyltransferase 3-like protein
• UniProt Protein Name: DNA (cytosine-5)-methyltransferase 3-like
• UniProt Gene Name: DNMT3L
• UniProt Entry Name: DNM3L_HUMAN

NCBI Description

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternative splicing results in two transcript variants. An additional splice variant has been described but its biological validity has not been determined. [provided by RefSeq]