DNMT3L recombinant protein

Recombinant DNMT3L protein

Cat. Num. AAA388132

• Gene Names: DNMT3L; MGC1090
• Purity: The recombinant protein is >80% pure by SDS-PAGE.
• Synonyms: DNMT3L; Recombinant DNMT3L protein; DNA Methyltransferase 3 Like; DNMT3L recombinant protein

• Host: Baculovirus
• Purity/Purification: The recombinant protein is >80% pure by SDS-PAGE.
• Form/Format: Full length recombinant DNMT3L protein is supplied in 25mM Tris pH8.0, 300mM NaCl, 5% Glycerol and 0.04% Triton X-100, 1mM DTT.

• Protein Species: Human
• Tag: His-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant DNMT3L protein gel. Recombinant DNMT3L protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.)

Related Product Information for DNMT3L recombinant protein

Short Description: Full-length recombinant DNMT3L protein (accession number NP_787063.1) was expressed in Sf9 cells and contains an N-terminal 6xHis-tag with a molecular weight of 46.6 kDa. The recombinant protein is >80% pure by SDS-PAGE. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Background: DNMT3L (DNA (Cytosine-5-)-Methyltransferase 3-Like) is a nuclear protein with similarity to the DNMT family of proteins, but does not function as a DNA methyltransferase. It serves as an essential regulator of DNMT3A and DNMT3B, recruiting them to sites for deposition of de novo DNA methylation. DNMT3L is expressed in the presumptive gametes in mammalian embryos and is required for the establishment of genomic imprinting. DNMT3L has a PHD-like domain that allows binding to chromatin through interactions with the amino terminal tail of histone H3 that is devoid of lysine 4 methylation.

Product Categories/Family for DNMT3L recombinant protein

Recombinant Proteins; DNA Methyltransferase (DNMT)

NCBI and Uniprot Product Information

• NCBI GI #: 28872780
• NCBI GeneID: 29947
• NCBI Accession #: NP_787063.1
• NCBI GenBank Nucleotide #: NM_175867.1
• UniProt Accession #: Q9UJW3; Q9BUJ4; E9PB42
• Molecular Weight: 43,583 Da
• NCBI Official Full Name: DNA (cytosine-5)-methyltransferase 3-like isoform 2
• NCBI Official Synonym Full Names: DNA (cytosine-5-)-methyltransferase 3-like
• NCBI Official Symbol: DNMT3L
• NCBI Official Synonym Symbols: MGC1090
• NCBI Protein Information: DNA (cytosine-5)-methyltransferase 3-like; DNA (cytosine-5)-methyltransferase 3-like; OTTHUMP00000109516; OTTHUMP00000109517; cytosine-5-methyltransferase 3-like protein; human cytosine-5-methyltransferase 3-like protein
• UniProt Protein Name: DNA (cytosine-5)-methyltransferase 3-like
• Protein Family: DNA (cytosine-5)-methyltransferase
• UniProt Gene Name: DNMT3L
• UniProt Entry Name: DNM3L_HUMAN

NCBI Description

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternative splicing results in two transcript variants. An additional splice variant has been described but its biological validity has not been determined. [provided by RefSeq]