DNMT3A recombinant protein

Recombinant DNMT3A protein

Cat. Num. AAA388124

• Gene Names: DNMT3A; TBRS; DNMT3A2; M.HsaIIIA
• Purity: The recombinant protein is >95% pure by SDS-PAGE.
• Synonyms: DNMT3A; Recombinant DNMT3A protein; TBRS; DNMT3A2; M.HsaIIIA; DNMT3A recombinant protein

• Host: Baculovirus
• Purity/Purification: The recombinant protein is >95% pure by SDS-PAGE.
• Form/Format: Recombinant DNMT3A protein is supplied in 25mM Tris pH8.0, 500mM NaCl, 5% Glycerol, 0.04% Triton X-100, 1mM DTT and 0.2mg/ml 3× peptide.

• Source: Human
• Tag: DYKDDDDK-Tag, His-Tag
• Notes: Recombinant DNMT3A protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.
• DNMT Activity Assay Conditions: DNMT Activity Assay Conditions: Assay was performed using DNMT Activity/Inhibition Assay, for 1 hour at 37 degree C.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Ships with Dry Ice.

SDS-Page

(Recombinant DNMT3A protein gel. Recombinant DNMT3A protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.)

Testing Data

(Recombinant DNMT3A protein activity assay Recombinant DNMT3A protein activity was measured using DNMT Activity / Inhibition Assay, for 1 hour at 37 degree C)

Related Product Information for DNMT3A recombinant protein

Background: (DNA (Cytosine-5-)-Methyltransferase 3) proteins such as DNMT3A (or DNMT 3A) are involved in DNA methylation, in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. DNMT3A has been shown to be important in the regulation of specific patterns of DNA methylation. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and control of gene expression. DNA methylation is generally associated with transcriptional repression.

Three families of DNMTs have been identified: DNMT1, DNMT2 and DNMT3. The DNMT3 family, containing two active methyltransferases, DNMT3A & DNMT3B, and one DNMT3-Like protein (DNMT3L), establishes the initial CpG methylation pattern de novo and shows the same propensity for methylating unmethylated duplex DNA as for hemi-methylated DNA. DNMT3A and DNMT3B are encoded by two distinct genes and are strongly expressed in embryonic stem cells. DNMT3A is ubiquitous in mammalian cells

Product Categories/Family for DNMT3A recombinant protein

Recombinant Proteins; DNA Methyltransferase (DNMT)

NCBI and Uniprot Product Information

• NCBI GI #: 28559069
• NCBI GeneID: 1788
• NCBI Accession #: NP_783328.1; NP_715640.2
• NCBI GenBank Nucleotide #: NM_175629.2
• UniProt Accession #: Q9Y6K1; Q86TE8; Q86XF5; Q8IZV0; Q8WXU9; E9PEB8
• Molecular Weight: 101858 Da
• NCBI Official Full Name: DNA (cytosine-5)-methyltransferase 3A isoform a
• NCBI Official Synonym Full Names: DNA (cytosine-5-)-methyltransferase 3 alpha
• NCBI Official Symbol: DNMT3A
• NCBI Official Synonym Symbols: TBRS; DNMT3A2; M.HsaIIIA
• NCBI Protein Information: DNA (cytosine-5)-methyltransferase 3A
• UniProt Protein Name: DNA (cytosine-5)-methyltransferase 3A
• Protein Family: DNA
• UniProt Gene Name: DNMT3A
• UniProt Synonym Gene Names: Dnmt3a; DNA MTase HsaIIIA; M.HsaIIIA
• UniProt Entry Name: DNM3A_HUMAN

NCBI Description

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. [provided by RefSeq, Mar 2016]