Canine Glutathione S Transferase Theta 2 ELISA Kit | GSTt2 elisa kit

Canine Glutathione S Transferase Theta 2 ELISA Kit

Cat. Num. AAA736157

• Gene Names: GSTA2; GST2; GTA2; GTH2; GSTA2-2
• Reactivity: Canine
• Synonyms: Glutathione S Transferase Theta 2; Canine Glutathione S Transferase Theta 2 ELISA Kit; GSTt2 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 5.0-100 U/L
• Sensitivity: 1.0U/L
• Intended Uses: This GSTT2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine GSTT2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GSTt2 elisa kit

Principle of the Assay: GSTT2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GSTT2 antibody and an GSTT2-HRP conjugate. The assay sample and buffer are incubated together with GSTT2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GSTT2 concentration since GSTT2 from samples and GSTT2-HRP conjugate compete for the anti-GSTT2 antibody binding site. Since the number of sites is limited, as more sites are occupied by GSTT2 from the sample, fewer sites are left to bind GSTT2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GSTT2 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 215276987
• NCBI GeneID: 2939
• NCBI Accession #: NP_000837.3
• NCBI GenBank Nucleotide #: NM_000846.4
• Molecular Weight: 25,664 Da
• NCBI Official Full Name: glutathione S-transferase A2
• NCBI Official Synonym Full Names: glutathione S-transferase alpha 2
• NCBI Official Symbol: GSTA2
• NCBI Official Synonym Symbols: GST2; GTA2; GTH2; GSTA2-2
• NCBI Protein Information: glutathione S-transferase A2; GST-gamma; liver GST2; GST HA subunit 2; GST class-alpha member 2; glutathione S-aryltransferase A2; glutathione S-alkyltransferase A2; glutathione S-aralkyltransferase A2; S-(hydroxyalkyl)glutathione lyase A2
• UniProt Protein Name: Glutathione S-transferase A2
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: GSTA2
• UniProt Synonym Gene Names: GST2
• UniProt Entry Name: GSTA2_HUMAN

NCBI Description

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding these enzymes are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of some drugs. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, located in a cluster mapped to chromosome 6, are the most abundantly expressed glutathione S-transferases in liver. In addition to metabolizing bilirubin and certain anti-cancer drugs in the liver, the alpha class of these enzymes exhibit glutathione peroxidase activity thereby protecting the cells from reactive oxygen species and the products of peroxidation. [provided by RefSeq, Jul 2008]

Uniprot Description

GSTA2: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Belongs to the GST superfamily. Alpha family.

Protein type: Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 2.5.1.18; Xenobiotic Metabolism - metabolism by cytochrome P450; Transferase; Other Amino Acids Metabolism - glutathione

Chromosomal Location of Human Ortholog: 6p12.1

Cellular Component: cytosol

Molecular Function: glutathione transferase activity

Biological Process: glutathione metabolic process; epithelial cell differentiation; xenobiotic metabolic process