Mouse anti-Human Eosinophil Peroxidase (EPX) Monoclonal Antibody | anti-EPX antibody

Eosinophil Peroxidase (EPX)

Cat. Num. AAA4381637

• Gene Names: EPX; EPO; EPP; EPXD; EPX-PEN
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Eosinophil Peroxidase (EPX); Monoclonal Antibody; Eosinophil peroxidase heavy chain; EPER; EPO; EPP; EPX; EPX PEN; PERE; anti-EPX antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: rEPO104
• Specificity: Peripheral blood granulocytes are classified into neutrophils, basophils and eosinophils according to the staining characteristics of their cytoplasmic granules. Granule proteins are released by physiologic and pharmacologic stimuli and play important roles in both normal and pathological host immune responses. Eosinophil major basic protein and eosinophil peroxidase (EPX) are granule proteins specific to the eosinophil. AHE-1 recognizes human EPX, a granule protein specific to eosinophils. It does not cross-react with eosinophil major basic protein, elastase, cathepsin G, esterase N, thrombin, plasmin, kallikrein, lactoferrin, or transferrin. This MAb stains eosinophils only and does not stain other peripheral blood cells, including platelets, neutrophils, monocytes, lymphocytes or red blood cells. Human EPX gene product can form a tetramer of two light chains and two heavy chains. Other peroxidase family members include myeloperoxidase (MPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO).
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 715

• Applicable Applications for anti-EPX antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin
• Application Notes: FC/FACS: 0.5-1ug/million cells; IF: 0.5-1ug/ml; IHC-P: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degree C followed by cooling at RT for 20 minutes
• Immunogen: Human eosinophils from a patient with hypereosinophilic syndrome
• Cellular Localization: Cytoplasmic and Granular
• Antibody Type: Recombinant Antibody
• Hu-chromosome Location: 17q22
• Positive Control: MCF-7 cells. Tonsil
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)-Formallin

(Formalin-fixed, paraffin-embedded human Pancreas stained with EPX Recombinant Mouse Monoclonal Antibody (rEPO104). )

SDS-Page

(SDS-PAGE Analysis Purified EPX Recombinant Mouse Monoclonal Antibody (rEPO104). Confirmation of Purity and Integrity of Antibody. )

References

Skubitz, K.M., et al. 1989. J. Leukocyte Biol. 46:109-118. | Skubitz, K.M., et al. 1988. J. leukocyte Bio. 44:158-165.

NCBI and Uniprot Product Information

• NCBI GI #: 1352738
• NCBI GeneID: 8288
• UniProt Accession #: P11678
• Molecular Weight: 70kDa (55kDa-Heavy Chain; 15kDa-Light Chain)
• NCBI Official Full Name: Eosinophil peroxidase
• NCBI Official Synonym Full Names: eosinophil peroxidase
• NCBI Official Symbol: EPX
• NCBI Official Synonym Symbols: EPO; EPP; EPXD; EPX-PEN
• NCBI Protein Information: eosinophil peroxidase
• UniProt Protein Name: Eosinophil peroxidase
• Protein Family: Eosinophil peroxidase
• UniProt Gene Name: EPX
• UniProt Synonym Gene Names: EPER; EPO; EPP; EPO
• UniProt Entry Name: PERE_HUMAN

NCBI Description

This gene is a member of the peroxidase gene family and is expressed in eosinophils. The encoded preproprotein is proteolytically processed into covalently attached heavy and light chains to form the mature enzyme, which functions as an oxidant. The enzyme is released at sites of parasitic infection or allergen stimulation to mediate lysis of protozoa or parasitic worms. The gene is found in a gene cluster with other peroxidase genes on chromosome 17. Mutations in this gene result in eosinophil peroxidase deficiency. [provided by RefSeq, Feb 2016]

Uniprot Description

EPX: Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. Shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis. Defects in EPX are the cause of eosinophil peroxidase deficiency (EPD). EPD is an autosomal recessive defect where anomalous eosinophils are characterized by nuclear hypersegmentation, hypogranulation, and negative peroxidase and phospholipid staining. Belongs to the peroxidase family. XPO subfamily.

Protein type: Oxidoreductase; EC 1.11.1.7

Chromosomal Location of Human Ortholog: 17q23.1

Molecular Function: peroxidase activity; metal ion binding; heme binding

Biological Process: positive regulation of interleukin-4 production; negative regulation of interleukin-10 production; hydrogen peroxide catabolic process; negative regulation of interleukin-5 production; response to oxidative stress; defense response to nematode

Disease: Eosinophil Peroxidase Deficiency