Testing Data
(DNMT1 activity assay. Recombinant DNMT1 activity was incubated with 400 uM S-adenosylmethionine (SAM) and DNA substrate, followed by detection with anti-5mC antibody and chemiluminescent detection.)
DNMT1 recombinant protein
Recombinant DNMT1 protein
Synonyms
DNMT1; Recombinant DNMT1 protein; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; DNMT1 recombinant protein
Host
Baculovirus
Form/Format
10ug recombinant DNMT1 protein supplied in 40mM Tris-HCl, pH8.0, 110mM NaCl, 2.2mM KCl, 0.04% Tween-20, and 20% glycerol.
Protein Species
Human
Tag
GST-Tag
Notes
Recombinant DNMT1 is suitable for use in DNA methylation assays. This protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Testing Data
(DNMT1 activity assay. Recombinant DNMT1 activity was incubated with 400 uM S-adenosylmethionine (SAM) and DNA substrate, followed by detection with anti-5mC antibody and chemiluminescent detection.)
Testing Data
(DNMT1 activity assay. Recombinant DNMT1 activity was incubated with 400 uM S-adenosylmethionine (SAM) and DNA substrate, followed by detection with anti-5mC antibody and chemiluminescent detection.)
SDS-Page
(DNMT1 protein gel. DNMT1 (2.8 ug) run on a 4-20% SDS-PAGE gel and stained with Coomassie Blue. Arrow indicates recombinant DNMT1.)
SDS-Page
(DNMT1 protein gel. DNMT1 (2.8 ug) run on a 4-20% SDS-PAGE gel and stained with Coomassie Blue. Arrow indicates recombinant DNMT1.)
Related Product Information for DNMT1 recombinant protein
Short Description: Recombinant human DNMT1 was expressed in Sf9 cells via a baculovirus expression system as amino acids 2-1632 (accession number NM_001130823) with an N-terminal GST-Tag. The molecular weight of the protein is 211 kDa. DNMT1-DNA methyltransferase 1 (or DNMT 1) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B). DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression. Recombinant DNMT1 can be used in DNA methylation assays to study enzyme kinetics and inhibitors.
Background: DNMT1-DNA methyltransferase 1 (or DNMT 1) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B). DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression.
Background: DNMT1-DNA methyltransferase 1 (or DNMT 1) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B). DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression.
Product Categories/Family for DNMT1 recombinant protein
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Molecular weight of the protein is 211kDa
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Product Notes
The DNMT1 (Catalog #AAA388081) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "DNMT1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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