SDS-Page
(Recombinant DNMT1 protein gel. Recombinant DNMT1 protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue. MW: 188.2 kDa Purity: > 90%)
DNMT1 recombinant protein
Recombinant DNMT1 protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant DNMT1 protein gel. Recombinant DNMT1 protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue. MW: 188.2 kDa Purity: > 90%)
SDS-Page
(Recombinant DNMT1 protein gel. Recombinant DNMT1 protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue. MW: 188.2 kDa Purity: > 90%)
Testing Data
(Recombinant DNMT1 protein activity assay Assay was performed using DNMT Activity / Inhibition Assay,. The substrate, AdoMet and DNMT1 were incubated for 1 hour at 37 degree C. The whole reactions were developed for 4 min. )
Testing Data
(Recombinant DNMT1 protein activity assay Assay was performed using DNMT Activity / Inhibition Assay,. The substrate, AdoMet and DNMT1 were incubated for 1 hour at 37 degree C. The whole reactions were developed for 4 min. )
Background: DNMT1 (DNA (Cytosine-5-)-Methyltransferase 1) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B). DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression.
NCBI and Uniprot Product Information
NCBI Description
DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
Uniprot Description
DNMT1: an ubiquitous DNA methyltransferase that methylates CpG residues. Preferentially methylates hemimethylated DNA. It is responsible for maintaining methylation patterns established in development, and may play an active role in DNA damage repair. Mediates transcriptional repression by direct binding to HDAC2. Its abundance is reduced to non detectable levels at the G0 phase of the cell cycle and is dramatically induced upon entrance into the S-phase of the cell cycle. Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Three isoforms of the human protein produced by alternative splicing have been described.
Protein type: Methyltransferase, DNA; EC 2.1.1.37; Cell development/differentiation; Methyltransferase; Transcription regulation; Amino Acid Metabolism - cysteine and methionine
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: nucleoplasm; centric heterochromatin; replication fork; nucleus
Molecular Function: DNA (cytosine-5-)-methyltransferase activity; protein binding; methyl-CpG binding; DNA binding; zinc ion binding; RNA binding; chromatin binding; DNA-methyltransferase activity; transcription factor binding
Biological Process: negative regulation of histone H3-K9 methylation; transcription, DNA-dependent; maintenance of DNA methylation; negative regulation of gene expression, epigenetic; DNA methylation; gene expression; negative regulation of transcription from RNA polymerase II promoter; positive regulation of histone H3-K4 methylation; chromatin modification; gene silencing; regulation of gene expression, epigenetic; DNA methylation on cytosine; regulation of cell proliferation
Disease: Cerebellar Ataxia, Deafness, And Narcolepsy, Autosomal Dominant; Neuropathy, Hereditary Sensory, Type Ie
Research Articles on DNMT1
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Product Notes
The DNMT1 dnmt1 (Catalog #AAA388122) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "DNMT1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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