SDS-PAGE
Dickkopf Related Protein 4 (DKK4) Recombinant Protein | DKK4 recombinant protein
Recombinant Dickkopf Related Protein 4 (DKK4)
Gene Names
Dkk4; Dkk-4
Applications
SDS-Page, Western Blot
Purity
> 95%
Synonyms
Dickkopf Related Protein 4 (DKK4); Recombinant Dickkopf Related Protein 4 (DKK4); DKK4 recombinant protein
Host
E.coli
Purity/Purification
> 95%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 600ug/mL (varies by lot)
Sequence
KGSL CASDRDCSEG KFCLAFHDERSFCATCRRVR RRCQRSAVCC PGTVCVNDVC TAVEDTRPVM DRNTDGQDGA YAEGTTKWPA EENRPQGKPS TKKSQSSKGQ E
Applicable Applications for DKK4 recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Organism Species
Mus musculus (Mouse)
Source
Prokaryotic expression
Residues
Lys37~Glu141 (Accession # Q8VEJ3)
Tags
N-terminal His and GST Tag
Subcellular Location
Secreted.
Traits
Freeze-dried powder
Predicted isoelectric point
6.5
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
SDS-PAGE
SDS-PAGE
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 43.5kDa
Accurate Molecular Mass: 50kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 50kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
dickkopf-related protein 4
NCBI Official Synonym Full Names
dickkopf homolog 4 (Xenopus laevis)
NCBI Official Symbol
Dkk4
NCBI Official Synonym Symbols
Dkk-4
NCBI Protein Information
dickkopf-related protein 4; dickkopf-4
UniProt Protein Name
Dickkopf-related protein 4
Protein Family
UniProt Gene Name
Dkk4
UniProt Synonym Gene Names
Dickkopf-4; Dkk-4
UniProt Entry Name
DKK4_MOUSE
Uniprot Description
DKK4: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Belongs to the dickkopf family.
Protein type: Secreted; Secreted, signal peptide; Inhibitor
Cellular Component: extracellular region
Biological Process: negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway; multicellular organismal development
Research Articles on DKK4
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Product Notes
The DKK4 dkk4 (Catalog #AAA2010241) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Dickkopf Related Protein 4 (DKK4) can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the DKK4 dkk4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KGSL CASDRDCSEG KFCLAFHDER SFCATCRRVR RRCQRSAVCC PGTVCVNDVC TAVEDTRPVM DRNTDGQDGA YAEGTTKWPA EENRPQGKPS TKKSQSSKGQ E. It is sometimes possible for the material contained within the vial of "Dickkopf Related Protein 4 (DKK4), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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