T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) Recombinant Protein | TIMD4 recombinant protein

Recombinant T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4)

Cat. Num. AAA2105614

• Gene Names: TIMD4; TIM4; SMUCKLER
• Reactivity: Homo sapiens (Human)
• Applications: SDS-Page, Western Blot
• Purity: > 90%
• Synonyms: T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4); Recombinant T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4); TIM4; T-cell membrane protein 4; T-cell immunoglobulin mucin receptor 4; TIMD4 recombinant protein

• Host: E. coli
• Reactivity: Homo sapiens (Human)
• Purity/Purification: > 90%
• Form/Format: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
• Concentration: Original Concentration: 80ug/mL (varies by lot)

• Applicable Applications for TIMD4 recombinant protein: Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
• Application Notes: (May be suitable for use in other assays to be determined by the end user.)
• Source: Prokaryotic expression
• Residues: Glu25~Gln314
• Tags: N-terminal His Tag
• Subcellular Location: Membrane
• Traits: Freeze-dried powder
• Predicted isoelectric point: 6.5
• Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:; 1. Splice variants: Alternative splicing may create different sized proteins from the same gene.; 2. Relative charge: The composition of amino acids may affects the charge of the protein.; 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.; 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.; 5. Polymerization of the target protein: Dimerization, multimerization etc.
• Usage: Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 2-8 degree C for one month. ; Aliquot and store at -80 degree C for 12 months.; ; Stability Test:; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Sequence

Gene Sequencing (Extract)

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 226529863
• NCBI GeneID: 91937
• NCBI Accession #: NP_612388.2
• NCBI GenBank Nucleotide #: NM_138379.3
• UniProt Accession #: Q96H15
• Molecular Weight: Predicted Molecular Mass: 35.0kDa; Accurate Molecular Mass: 40kDa as determined by SDS-PAGE reducing conditions.
• NCBI Official Full Name: T-cell immunoglobulin and mucin domain-containing protein 4 isoform 1
• NCBI Official Synonym Full Names: T cell immunoglobulin and mucin domain containing 4
• NCBI Official Symbol: TIMD4
• NCBI Official Synonym Symbols: TIM4; SMUCKLER
• NCBI Protein Information: T-cell immunoglobulin and mucin domain-containing protein 4
• UniProt Protein Name: T-cell immunoglobulin and mucin domain-containing protein 4
• Protein Family: T-cell immunoglobulin and mucin domain-containing protein
• UniProt Gene Name: TIMD4
• UniProt Synonym Gene Names: TIM4; TIMD-4; TIM-4
• UniProt Entry Name: TIMD4_HUMAN

Uniprot Description

TIM-4: Phosphatidylserine receptor that enhances the engulfment of apoptotic cells. Involved in regulating T-cell proliferation and lymphotoxin signaling. Ligand for HAVCR1/TIMD1. Belongs to the immunoglobulin superfamily. TIM family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q33.3

Cellular Component: integral to membrane