Testing Data
(Analysis of apoptosis-positive cells stained with Annexin V-FITC and PI. )
Annexin V-FITC Apoptosis Detection Kit
Annexin V-FITC Apoptosis Detection Kit
Synonyms
Annexin V-FITC Apoptosis; Annexin V-FITC Apoptosis Detection Kit; Annexin V-FITC Apoptosis detection kit
Samples
Cell culture
Target
Apoptosis, Exposed phosphatidyl serine
Excitation/Emission
488 nm / 530 nm
Reagent
Annexin V-FITC
Preparation and Storage
Store at 2-8 degree C.
Testing Data
(Analysis of apoptosis-positive cells stained with Annexin V-FITC and PI. )
Testing Data
(Analysis of apoptosis-positive cells stained with Annexin V-FITC and PI. )
Related Product Information for Annexin V-FITC Apoptosis detection kit
Description: Detect apoptosis and membrane permability with the Annexin V-FITC Apoptosis Assay Kit. This in vitro apoptosis assay employs the green fluorescent Annexin V-FITC reagent to label exposed phosphatidylserine in cultured cell samples. Detect membrane compromised cells, a trademark of late apoptosis or cell necrosis, with the live/dead stain, propidium iodide. Analyze the fluorescent signals by flow cytometry.
Background: Annexin V is a member of a calcium and phospholipid binding family of proteins with vascular anticoagulant activity. Results from in vitro experiments indicate that it may play a role in the inhibition of blood coagulation by competing for phosphatidylserine (PS) binding sites with prothrombin. In healthy cells, PS is usually kept in the inner-leaflet (the cytosolic side) of the cell membrane. When a cell undergoes apoptosis, one of the earliest detectable indicators is the loss of membrane asymmetry. No longer restricted to the cytosolic part of the membrane, PS is translocated to the outer-leaflet and becomes exposed on the surface of the cell. With our Annexin V-FITC Apoptosis Assay Kit, ImmunoChemistry Technologies provides a proven method for quickly and easily distinguishing two populations of dying cells from viable cells using Annexin V-FITC and Propidium Iodide (PI) reagents. Cells in the early stages of apoptosis with intact cell membranes and surface -exposed PS will stain positive for Annexin V-FITC. PI is included in the apoptosis assay kit to identify late apoptotic and necrotic cells, which have lost plasma membrane integrity. These cells will become dually labeled with Annexin V-FITC and Propidium Iodide (green and red fluorescence). Live cells with intact plasma membranes will exclude PI and will remain unstained by the Annexin V-FITC probe, assuming no treatment or cell cycle-associated event temporarily exposes the normally internalized, negatively charged PS entity. The kit also includes a specially formulated, calcium-based binding buffer, which is required for Annexin V binding to occur.
Background: Annexin V is a member of a calcium and phospholipid binding family of proteins with vascular anticoagulant activity. Results from in vitro experiments indicate that it may play a role in the inhibition of blood coagulation by competing for phosphatidylserine (PS) binding sites with prothrombin. In healthy cells, PS is usually kept in the inner-leaflet (the cytosolic side) of the cell membrane. When a cell undergoes apoptosis, one of the earliest detectable indicators is the loss of membrane asymmetry. No longer restricted to the cytosolic part of the membrane, PS is translocated to the outer-leaflet and becomes exposed on the surface of the cell. With our Annexin V-FITC Apoptosis Assay Kit, ImmunoChemistry Technologies provides a proven method for quickly and easily distinguishing two populations of dying cells from viable cells using Annexin V-FITC and Propidium Iodide (PI) reagents. Cells in the early stages of apoptosis with intact cell membranes and surface -exposed PS will stain positive for Annexin V-FITC. PI is included in the apoptosis assay kit to identify late apoptotic and necrotic cells, which have lost plasma membrane integrity. These cells will become dually labeled with Annexin V-FITC and Propidium Iodide (green and red fluorescence). Live cells with intact plasma membranes will exclude PI and will remain unstained by the Annexin V-FITC probe, assuming no treatment or cell cycle-associated event temporarily exposes the normally internalized, negatively charged PS entity. The kit also includes a specially formulated, calcium-based binding buffer, which is required for Annexin V binding to occur.
Product Categories/Family for Annexin V-FITC Apoptosis detection kit
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Product Notes
The Annexin V-FITC Apoptosis (Catalog #AAA258045) is a Detection Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Annexin V-FITC Apoptosis, Detection Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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